.sub may refer to:
- MicroDVD Subtitle File, a file that contains subtitle (captioning) data for a movie or video clip
- Subchannel Data File, a file that can be a part of CloneCD image files; see CloneCD Control File
- DirectVobSub Subtitle File, a binary file that contains subtitles (captioning) data for a movie or video clip
This disambiguation page lists articles associated with the title .sub.
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