6-Hydroxypseudooxynicotine dehydrogenase

6-hydroxypseudooxynicotine dehydrogenase
6-hydroxypseudooxynicotine dehydrogenase
Identifiers
EC no.	1.5.99.14
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
PMC
Search
PMC	articles
PubMed	articles
NCBI	proteins

6-hydroxypseudooxynicotine dehydrogenase (EC 1.5.99.14) is an enzyme with systematic name 1-(6-hydroxypyridin-3-yl)-4-(methylamino)butan-1-one:acceptor 6-oxidoreductase (hydroxylating).^[1]^[2]^[3] This enzyme catalyses the following chemical reaction:

1-(6-hydroxypyridin-3-yl)-4-(methylamino)butan-1-one + acceptor + H₂O

\rightleftharpoons

1-(2,6-dihydroxypyridin-3-yl)-4-(methylamino)butan-1-one + reduced acceptor

This enzyme contains a cytidylyl molybdenum cofactor.

References

^ Freudenberg W, Konig K, Andreesen JR (1988). "Nicotine dehydrogenase from Arthrobacter oxidans: A molybdenum-containing hydroxylase". FEMS Microbiology Letters. 52: 13–18. doi:10.1111/j.1574-6968.1988.tb02564.x.
^ Grether-Beck S, Igloi GL, Pust S, Schilz E, Decker K, Brandsch R (September 1994). "Structural analysis and molybdenum-dependent expression of the pAO1-encoded nicotine dehydrogenase genes of Arthrobacter nicotinovorans". Molecular Microbiology. 13 (5): 929–36. doi:10.1111/j.1365-2958.1994.tb00484.x. PMID 7815950.
^ Sachelaru P, Schiltz E, Brandsch R (July 2006). "A functional mobA gene for molybdopterin cytosine dinucleotide cofactor biosynthesis is required for activity and holoenzyme assembly of the heterotrimeric nicotine dehydrogenases of Arthrobacter nicotinovorans". Applied and Environmental Microbiology. 72 (7): 5126–31. doi:10.1128/AEM.00437-06. PMC 1489357. PMID 16820521.