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In enzymology, a 7alpha-hydroxysteroid dehydrogenase (EC 1.1.1.159) is an enzyme that catalyzes the chemical reaction
| 7-alpha-hydroxysteroid dehydrogenase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC no. | 1.1.1.159 | ||||||||
| CAS no. | 39361-64-3 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| Gene Ontology | AmiGO / QuickGO | ||||||||
| |||||||||
- 3alpha,7alpha,12alpha-trihydroxy-5beta-cholanate + NAD+ 3alpha,12alpha-dihydroxy-7-oxo-5beta-cholanate + NADH + H+
Thus, the two substrates of this enzyme are 3alpha,7alpha,12alpha-trihydroxy-5beta-cholanate and NAD+, whereas its 3 products are 3alpha,12alpha-dihydroxy-7-oxo-5beta-cholanate, NADH, and H+.
This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with NAD+ or NADP+ as acceptor. The systematic name of this enzyme class is 7alpha-hydroxysteroid:NAD+ 7-oxidoreductase. Other names in common use include 7alpha-hydroxy steroid dehydrogenase, and 7alpha-HSDH.
Structural studies
editAs of late 2007, 3 structures have been solved for this class of enzymes, with PDB accession codes 1AHH, 1AHI, and 1FMC.
References
edit- Haslewood ES, Haslewood GA (July 1976). "The specificity of a 7 alpha-hydroxy steroid dehydrogenase from Escherichia coli". The Biochemical Journal. 157 (1): 207–10. doi:10.1042/bj1570207. PMC 1163832. PMID 786279.
- MacDonald IA, Roach PD (August 1981). "Bile induction of 7 alpha- and 7 beta-hydroxysteroid dehydrogenases in Clostridium absonum". Biochimica et Biophysica Acta. 665 (2): 262–9. doi:10.1016/0005-2760(81)90011-4. PMID 6945134.
- Macdonald IA, Williams CN, Mahony DE (June 1973). "7Alpha-hydroxysteroid dehydrogenase from Escherichia coli B: preliminary studies". Biochimica et Biophysica Acta (BBA) - Enzymology. 309 (2): 243–53. doi:10.1016/0005-2744(73)90022-3. PMID 4581498.
- Macdonald IA, Williams CN, Mahony DE, Christie WM (March 1975). "NAD- and NADP-dependent 7alpha-hydroxysteroid dehydrogenases from bacteroides fragilis". Biochimica et Biophysica Acta (BBA) - Enzymology. 384 (1): 12–24. doi:10.1016/0005-2744(75)90091-1. PMID 236764.
