AEN is may refer to:
- Acute esophageal necrosis, a rare esophageal disorder
- A+E Networks, a media organization based in the United States
- AEN Ayiou Georgiou Vrysoullon-Acheritou, an association football club in the Agios Georgios refugee settlement in Cyprus
- Alliance for Europe of the Nations, a pan-European political party
- Alfred E. Neuman, a fictional character in MAD magazine
- Armenian Environmental Network
- Armenian Sign Language
- asynchronous event notification in (for example) the NC-SI electrical interface