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Acanthamoeba

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Acanthamoeba is a genus of amoebae that are commonly recovered from soil, fresh water, and other habitats. The genus Acanthamoeba has two stages in its life cycle, the metabolically active trophozoite stage and a dormant, stress-resistant cyst stage. In nature, Acanthamoeba species are generally free-living bacterivores. However, they are also opportunistic pathogens able to cause serious and sometimes fatal infections in humans and other animals.[1]

Acanthamoeba
Phase contrast micrograph of an Acanthamoeba polyphaga cyst.
Scientific classification Edit this classification
Domain: Eukaryota
Phylum: Amoebozoa
Class: Discosea
Order: Centramoebida
Family: Acanthamoebidae
Genus: Acanthamoeba
Volkonsky 1931
Type species
Acanthamoeba castellanii
Volkonsky 1931

Distribution

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Acanthamoeba spp. are among the most prevalent protozoa found in the environment.[1] They are distributed worldwide, and have been isolated from soil, air, sewage, seawater, chlorinated swimming pools, domestic tap water, bottled water, dental treatment units, hospitals, air-conditioning units, and contact lens cases. Additionally, they have been isolated from human skin, nasal cavities, throats, and intestines, as well as plants and other mammals.[2]

Role in disease

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Acanthamoeba encephalitis microscopy and molecular diagnostics: scale bar: 10 μm

Diseases caused by Acanthamoeba include keratitis and granulomatous amoebic encephalitis (GAE).[3] The latter is often but not always seen in immunosuppressed patients.[4] GAE is caused by the amoebae entering the body through an open wound and then spreading to the brain.[5] The combination of host immune responses and secreted amoebal proteases causes massive brain swelling[6] resulting in death in about 95% of those infected.

Granulomatous amoebic encephalitis (GAE)

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Granulomatous amoebic encephalitis (GAE) is caused by amoebic infection of the central nervous system (CNS). It is characterized by neurological symptoms including headache, seizures, and mental-status abnormalities.[1] These worsen progressively over weeks to months, leading to death in most patients.[1] Infection is generally associated with underlying conditions such as immunodeficiency, diabetes, malignancies, malnutrition, systemic lupus erythematosus, and alcoholism.[1] The parasite enters the body through cuts in the skin or by being inhaled into the upper respiratory tract.[1] The parasite then spreads through the blood into the CNS. Acanthamoeba crosses the blood–brain barrier by means that are not yet understood. Subsequent invasion of the connective tissue and induction of pro-inflammatory responses leads to neuronal damage that can be fatal within days. Pure granulomatous lesions are rare in patients with AIDS and other related immunodeficiency states, as the patients do not have adequate numbers of CD+ve T-cells to mount a granulomatous response to Acanthamoeba infection in CNS and other organs and tissues.[4] A perivascular cuffing with amoebae in necrotic tissue is usual finding in the AIDS and related T-cell immunodeficiency conditions.

Brain biopsy normally reveals severe oedema and hemorrhagic necrosis.[7] A patient who has contracted this illness usually displays subacute symptoms, including altered mental status, headaches, fever, neck stiffness, seizures, and focal neurological signs (such as cranial nerve palsies and coma), all leading to death within one week to several months.[8] Due to the rarity of this parasite and a lack of knowledge, no good diagnoses or treatments for Acanthamoeba infection are now known. Acanthamoeba keratitis cases in the past have resolved from a therapy consisting of atropine and some other drugs with no antimicrobial effects. Recent publications show atropine to interfere with the protist's CHRM1 receptor, causing cell death.[9]

Infection usually mimics that of bacterial leptomeningitis, tuberculous meningitis, or viral encephalitis. The misdiagnosis often leads to erroneous, ineffective treatment. In the case that the Acanthamoeba is diagnosed correctly, the current treatments, such as amphotericin B, rifampicin, trimethoprim-sulfamethoxazole, ketoconazole, fluconazole, sulfadiazine, or albendazole, are only tentatively successful. Correct and timely diagnosis, as well as improved treatment methods and an understanding of the parasite, are important factors in improving the outcome of infection by Acanthamoeba. A paper published in 2013 has shown substantial effects of some FDA-approved drugs with an in vitro kill rate above 90%.[4] These results were in vitro effects, but as the drugs are already approved, human infections can be targeted after dose calculations in clinical trials done with these diverse groups of drugs.

Acanthamoebic keratitis

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Pathogenic Acanthamoeba as seen under a light microscope

When present in the eye, Acanthamoeba strains can cause acanthamoebic keratitis, which may lead to corneal ulcers or even blindness.[10] This condition occurs most often among contact lens wearers who do not properly disinfect their lenses, exacerbated by a failure to wash hands prior to handling the lenses. Multipurpose contact lens solutions are largely ineffective against Acanthamoeba, whereas hydrogen peroxide-based solutions have good disinfection characteristics.[11][12]

The first cure of a corneal infection was achieved in 1985 at Moorfields Eye Hospital.[13]

In May 2007, Advanced Medical Optics, manufacturer of Complete Moisture Plus Contact Lens Solution products, issued a voluntary recall of their Complete Moisture Plus solutions. The fear was that contact lens wearers who used their solution were at higher risk of acanthamoebic keratitis than contact lens wearers who used other solutions. The manufacturer recalled the product after the Centers for Disease Control in the United States found that 21 people had possibly received an Acanthamoeba infection after using Complete Moisture Plus in the month prior to diagnosis.[14]

As a bacterial reservoir

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Several species of bacteria that can cause human disease are also able to infect and replicate within Acanthamoeba species.[1] These include Legionella pneumophila, Pseudomonas aeruginosa, and some strains of Escherichia coli and Staphylococcus aureus.[1][15] For some of these bacteria, replication inside Acanthamoeba has been associated with enhanced growth in macrophages, and increased resistance to some antibiotics.[1] Furthermore, due to the high prevalence of Acanthamoeba in the environment, these amoebae have been proposed to serve as an environmental reservoir for some human pathogens.[1]

Ecology

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A. castellanii can be found at high densities in various soil ecosystems. It preys on bacteria, but also fungi and other protozoa.

This species is able to lyse bacteria and produce a wide range of enzymes, such as cellulases or chitinases,[16] and probably contributes to the breakdown of organic matter in soil, contributing to the microbial loop.

Physiology

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Role as a model organism

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Because Acanthamoeba does not differ greatly at the ultrastructural level from a mammalian cell, it is an attractive model for cell-biology studies; it is important in cellular microbiology, environmental biology, physiology, cellular interactions, molecular biology, biochemistry, and evolutionary studies, due to the organisms' versatile roles in the ecosystem and ability to capture prey by phagocytosis, act as vectors and reservoirs for microbial pathogens, and to produce serious human infections. In addition, Acanthamoeba has been used extensively to understand the molecular biology of cell motility[17] and cancer cell dormancy by in-depth exploration of the process of encystation.[18]

The recently available Acanthamoeba genome sequence revealed several orthologs of genes employed in meiosis of sexual eukaryotes. These genes included Spo11, Mre11, Rad50, Rad51, Rad52, Mnd1, Dmc1, Msh, and Mlh.[19] This finding suggests that Acanthamoeba is capable of some form of meiosis and may be able to undergo sexual reproduction. Furthermore, since Acanthamoeba diverged early from the eukaryotic family tree, these results suggest that meiosis was present early in eukaryotic evolution.

Owing to its ease and economy of cultivation, the Neff strain of A. castellanii, discovered in a pond in Golden Gate Park in the 1960s, has been effectively used as a classic model organism in the field of cell biology. From just 30 L of simple medium inoculated with A. castellanii, about 1 kg of cells can be obtained after several days of aerated culture at room temperature. Pioneered in the laboratory of Edward D. Korn at the National Institutes of Health (NIH), many important biological molecules have been discovered and their pathways elucidated using the Acanthamoeba model. Thomas Dean Pollard applied this model at the NIH, Harvard Medical School, Johns Hopkins University School of Medicine, and the Salk Institute for Biological Studies to discover and characterize many proteins that are essential for cell motility, not only in amoebae, but also in many other eukaryotic cells, especially those of the human nervous and immune systems, the developing embryo, and cancer cells. Acanthamoeba also has served as a model to study the evolution of certain G-proteins. This unicellular eukaryote expresses few GPCRs over its cell membrane that serve vital role for the microorganism, structural homology bioinformatics tools have been used to show the presence of a homolog of human M1-muscarinic receptor in A. castellanii.[20] Blocking these muscarinic receptors in past studies has proven to be amoebicidal in Acanthamoeba spp.[5] More recently, voltage-gated calcium channels in Acanthamoeba spp. (CavAc) have been reported to have similarities with human voltage-gated calcium channels such as TPC-1 and L-type calcium channels and respond to Ca-channel blockers such as loperamide.[21] This model microbe has been studied to understand complex neurodegenerative states including Alzheimer's disease. Scientists have isolated a neurotransmitter acetylcholine in Acanthamoeba and the enzymatic machinery needed for its synthesis.[22]

Endosymbionts

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Acanthamoeba spp. contain diverse bacterial endosymbionts that are similar to human pathogens, so they are considered to be potential emerging human pathogens.[23] The exact nature of these symbionts and the benefit they represent for the amoebic host still have to be clarified. These include Legionella and Legionella-like pathogens.[24]

Giant viruses

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The giant viruses Mimivirus, Megavirus, and Pandoravirus infect Acanthamoeba.[25]

Members of the genus Acanthamoeba are unusual in serving as hosts for a variety of giant viruses (that have more than 1000 protein-coding genes; for instance, Pandoravirus, which has about 2500 protein-coding genes in its genome).

Diversity

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Acanthamoeba can be distinguished from other genera of amoebae based on morphological characteristics.[26] However, differentiating one species of Acanthamoeba from another by morphology has proven difficult. Based on 18S rDNA sequencing, known Acanthamoeba strains can be organized into 12 groups, denoted T1-T12.[26] Most disease-causing isolates belong to type T4.[26]

Below is a list of described species of Acanthamoeba, with sequence types noted where known. Species that have been identified in diseased patients are marked with *.

  • A. astronyxis (Ray & Hayes 1954) Page 1967 * (T7)
  • A. byersi Qvarnstrom, Nerad & Visvesvara 2013 *
  • A. castellanii Volkonski 1931 * (T4) [A. terricola Pussard 1964]
  • A. comandoni Pussard 1964 (T9)
  • A. culbertsoni (Singh & Das 1970) Griffin 1972 * (T10)
  • A. divionensis Pussard & Pons 1977 (T4)
  • A. echinulata Pussard & Pons 1977
  • A. gigantea Schmöller 1964
  • A. glebae (Dobell 1914)
  • A. gleichenii Volkonsky 1931
  • A. griffini Sawyer 1971 (T3)
  • A. hatchetti Sawyer, Visvesvara & Harke 1977 * (T11)
  • A. healyi Moura, Wallace & Visvesvara 1992 (T12)
  • A. hyalina Dobel & O'connor 1921
  • A. jacobsi Sawyer, Nerad & Visvesvara 1992
  • A. keratitis *
  • A. lenticulata Molet & Ermolieff-braun 1976 (T3)
  • A. lugdunensis Pussard & Pons 1977 * (T4)
  • A. mauritaniensis Pussard & Pons 1977 (T4)
  • A. micheli Corsaro et al. 2015
  • A. palestinensis (Reich 1933) Page 1977 * (T1)
  • A. paradivionensis Pussard & Pons 1977 (T4)
  • A. pearcei Nerad et al. 1995
  • A. polyphaga (Puschkarew 1913) Volkonsky 1931 * (T4)
  • A. pustulosa Pussard & Pons 1977 (T2)
  • A. pyriformis (Olive & Stoianovitch 1969) Spiegel & Shadwick 2016
  • A. quina Pussard & Pons 1977 *
  • A. rhysodes (Singh 1952) Griffin 1972 * (T4)
  • A. royreba Willaert, Stevens & Tyndall 1978
  • A. sohi Kyung-il & Shin 2003
  • A. stevensoni Sawyer et al. 1993 (T11)
  • A. triangularis Pussard & Pons 1977 (T4)
  • A. tubiashi Lewis & Sawyer 1979 (T8)

Etymology

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From the Greek akantha (spike/thorn), which was added before "amoeba" (change) to describe this organism as having a spine-like structure (acanthopodia). This organism is now well known as Acanthamoeba, an amphizoic, opportunistic, and nonopportunistic protozoan protist widely distributed in the environment.[27]

See also

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References

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  1. ^ a b c d e f g h i j Marciano-Cabral F, Cabral G (April 2003). "Acanthamoeba spp. as agents of disease in humans". Clinical Microbiology Reviews. 16 (2): 273–307. doi:10.1128/CMR.16.2.273-307.2003. PMC 153146. PMID 12692099.
  2. ^ De Jonckheere JF (1991). "Ecology of Acanthamoeba". Reviews of Infectious Diseases. 13 (Suppl 5): S385–7. doi:10.1093/clind/13.supplement_5.s385. PMID 2047667.
  3. ^ Di Gregorio C, Rivasi F, Mongiardo N, De Rienzo B, Wallace S, Visvesvara GS (December 1992). "Acanthamoeba meningoencephalitis in a patient with acquired immunodeficiency syndrome". Archives of Pathology & Laboratory Medicine. 116 (12): 1363–5. PMID 1456885.
  4. ^ a b c Baig AM (December 2014). "Granulomatous amoebic encephalitis: ghost response of an immunocompromised host?". Journal of Medical Microbiology. 63 (Pt 12): 1763–6. doi:10.1099/jmm.0.081315-0. PMID 25239626. S2CID 28069984.
  5. ^ a b Baig AM, Iqbal J, Khan NA (August 2013). "In vitro efficacies of clinically available drugs against growth and viability of an Acanthamoeba castellanii keratitis isolate belonging to the T4 genotype". Antimicrobial Agents and Chemotherapy. 57 (8): 3561–7. doi:10.1128/AAC.00299-13. PMC 3719691. PMID 23669391.
  6. ^ Baig AM (August 2015). "Pathogenesis of amoebic encephalitis: Are the amoebae being credited to an 'inside job' done by the host immune response?". Acta Tropica. 148: 72–6. doi:10.1016/j.actatropica.2015.04.022. PMID 25930186.
  7. ^ Khan NA (February 2007). "Acanthamoeba invasion of the central nervous system". International Journal for Parasitology. 37 (2): 131–8. doi:10.1016/j.ijpara.2006.11.010. PMID 17207487.
  8. ^ Kaushal V, Chhina DK, Kumar R, Pannu HS, Dhooria HP, Chhina RS (March 2007). "Acanthamoeba encephalitis". Indian Journal of Medical Microbiology. 26 (2): 182–4. doi:10.1016/S0255-0857(21)01941-1. hdl:1807/53578. PMID 18445961.
  9. ^ Baig AM, Zuberi H, Khan NA (May 2014). "Recommendations for the management of Acanthamoeba keratitis". Journal of Medical Microbiology. 63 (Pt 5): 770–1. doi:10.1099/jmm.0.069237-0. PMID 24509420.
  10. ^ Lorenzo-Morales J, Khan NA, Walochnik J (2015). "An update on Acanthamoeba keratitis: diagnosis, pathogenesis and treatment". Parasite. 22: 10. doi:10.1051/parasite/2015010. PMC 4330640. PMID 25687209.  
  11. ^ Shoff ME, Joslin CE, Tu EY, Kubatko L, Fuerst PA (July 2008). "Efficacy of contact lens systems against recent clinical and tap water Acanthamoeba isolates". Cornea. 27 (6): 713–9. doi:10.1097/QAI.0b013e31815e7251. PMID 18580265. S2CID 54503941.
  12. ^ Johnston SP, Sriram R, Qvarnstrom Y, Roy S, Verani J, Yoder J, Lorick S, Roberts J, Beach MJ, Visvesvara G (July 2009). "Resistance of Acanthamoeba cysts to disinfection in multiple contact lens solutions". Journal of Clinical Microbiology. 47 (7): 2040–5. doi:10.1128/JCM.00575-09. PMC 2708465. PMID 19403771.
  13. ^ Wright P, Warhurst D, Jones BR (October 1985). "Acanthamoeba keratitis successfully treated medically". The British Journal of Ophthalmology. 69 (10): 778–82. doi:10.1136/bjo.69.10.778. PMC 1040738. PMID 4052364.
  14. ^ "Abbott Medical Optics" (PDF). Archived from the original (PDF) on 7 July 2011. Retrieved 31 May 2009.
  15. ^ Huws SA, Morley RJ, Jones MV, Brown MR, Smith AW (May 2008). "Interactions of some common pathogenic bacteria with Acanthamoeba polyphaga". FEMS Microbiology Letters. 282 (2): 258–65. doi:10.1111/j.1574-6968.2008.01123.x. PMID 18399997.
  16. ^ Anderson IJ, Watkins RF, Samuelson J, Spencer DF, Majoros WH, Gray MW, Loftus BJ (August 2005). "Gene discovery in the Acanthamoeba castellanii genome". Protist. 156 (2): 203–14. doi:10.1016/j.protis.2005.04.001. PMID 16171187.
  17. ^ Khan N (2009). Acanthamoeba: Biology and Pathogenesis. Caister Academic Press. ISBN 978-1-904455-43-1.
  18. ^ Baig AM, Khan NA, Abbas F. Eukaryotic cell encystation and cancer cell dormancy: is a greater devil veiled in the details of a lesser evil? Cancer Biol
  19. ^ Khan NA, Siddiqui R (June 2015). "Is there evidence of sexual reproduction (meiosis) in Acanthamoeba?". Pathogens and Global Health. 109 (4): 193–5. doi:10.1179/2047773215Y.0000000009. PMC 4530557. PMID 25800982.
  20. ^ Baig AM, Ahmad HR (June 2017). "1-muscarinic GPCR homolog in unicellular eukaryotes: featuring Acanthamoeba spp bioinformatics 3D-modelling and experimentations". Journal of Receptor and Signal Transduction Research. 37 (3): 267–275. doi:10.1080/10799893.2016.1217884. PMID 27601178. S2CID 5234123.
  21. ^ Baig AM, Rana Z, Mannan M, Tariq S, Ahmad HR (2017). "Antibiotic Effects of Loperamide: Homology of Human Targets of Loperamide with Targets in Acanthamoeba spp". Recent Patents on Anti-Infective Drug Discovery. 12 (1): 44–60. doi:10.2174/1574891X12666170425170544. PMID 28506204.
  22. ^ Baig AM, Rana Z, Tariq S, Lalani S, Ahmad HR (March 2018). "Traced on the Timeline: Discovery of Acetylcholine and the Components of the Human Cholinergic System in a Primitive Unicellular Eukaryote Acanthamoeba spp". ACS Chemical Neuroscience. 9 (3): 494–504. doi:10.1021/acschemneuro.7b00254. PMID 29058403.
  23. ^ Horn M, Wagner M (September–October 2004). "Bacterial endosymbionts of free-living amoebae". The Journal of Eukaryotic Microbiology. 51 (5): 509–14. doi:10.1111/j.1550-7408.2004.tb00278.x. PMID 15537084. S2CID 21052932.
  24. ^ Schuster FL, Visvesvara GS (February 2004). "Opportunistic amoebae: challenges in prophylaxis and treatment". Drug Resistance Updates. 7 (1): 41–51. doi:10.1016/j.drup.2004.01.002. PMID 15072770.
  25. ^ Philippe N, Legendre M, Doutre G, Couté Y, Poirot O, Lescot M, Arslan D, Seltzer V, Bertaux L, Bruley C, Garin J, Claverie JM, Abergel C (July 2013). "Pandoraviruses: amoeba viruses with genomes up to 2.5 Mb reaching that of parasitic eukaryotes" (PDF). Science. 341 (6143): 281–6. Bibcode:2013Sci...341..281P. doi:10.1126/science.1239181. PMID 23869018. S2CID 16877147.
  26. ^ a b c Kong HH (October 2009). "Molecular phylogeny of acanthamoeba". The Korean Journal of Parasitology. 47 Suppl (Suppl): S21–8. doi:10.3347/kjp.2009.47.S.S21. PMC 2769217. PMID 19885332.
  27. ^ Pradhan, Nitika (August 2020). "Etymologia: Acanthamoeba". Emerg Infect Dis. 26 (8): 1855. doi:10.3201/eid2608.et2608. PMC 7392430. citing public domain text from the CDC
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