Anne or Ann Lee may refer to:
- Ann Lee (1736–1784), American religious leader known as "Mother Ann Lee"
- Ann Lee (activist) (born 1929 or 1930), American cannabis activist
- Ann Lee (actress) (1918–2003) American businesswoman and actress
- Ann Lee (illustrator) (1753–c. 1790), British natural history illustrator
- Anne Wharton (1659–1685), née Lee, English writer
- Anne Lee, Countess of Rochester (1585–1648)
- Ann Lee (singer) (born 1967), British Eurodance singer
- Ann Lee (professor), Chinese-American professor and author
- Anne Carter Lee (1839–1862), daughter of General Robert E. Lee