2-Carboxy-D-arabinitol-1-phosphatase

(Redirected from CA1P-phosphatase)

The enzyme 2-carboxy-D-arabinitol-1-phosphatase (CA1Pase; EC 3.1.3.63) catalyzes the reaction

2-Carboxy-D-arabinitol-1-phosphatase
Identifiers
EC no.3.1.3.63
CAS no.122319-88-4
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BRENDABRENDA entry
ExPASyNiceZyme view
KEGGKEGG entry
MetaCycmetabolic pathway
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Gene OntologyAmiGO / QuickGO
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NCBIproteins
2-carboxy-D-arabinitol 1-phosphate + H2O 2-carboxy-D-arabinitol + phosphate

This enzyme belongs to the family of hydrolases, to be specific, those acting on phosphoric monoester bonds. The systematic name is 2-carboxy-D-arabinitol-1-phosphate 1-phosphohydrolase.

In biology

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The best-studied 2-Carboxy-D-arabinitol-1-phosphate phosphatase is the enzyme that inactivates the RuBisCO inhibitor 2-carboxy-D-arabinitol-1-phosphate (CA1P).

When light levels are high, the inactivation occurs after CA1P has been released from RuBisCO by RuBisCO activase. As CA1P is present in many but not all plants, CA1P-mediated regulation of RuBisCO is not universal for all photosynthetic life. Amino acid sequences of the CA1Pase enzymes from wheat, French bean, tobacco, and Arabidopsis thaliana reveal that the enzymes contain 2 different domains, indicating that it is a multifunctional enzyme.

CA1Pase enzyme activity varies between different species due to their regulation by different redox-active compounds, such as glutathione. However, it is yet to be determined whether this process occurs in vivo. Wheat CA1Pase heterologously expressed in E. coli is also able to dephosphorylate the RuBisCO inhibitor D-glycero-2,3-diulose-1,5-bisphosphate.[1]

References

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  1. ^ Andralojc PJ, Madgwick PJ, Tao Y, Keys A, Ward JL, Beale MH, et al. (March 2012). "2-Carboxy-D-arabinitol 1-phosphate (CA1P) phosphatase: evidence for a wider role in plant Rubisco regulation". The Biochemical Journal. 442 (3): 733–42. doi:10.1042/BJ20111443. PMID 22132794.

Further reading

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