Coenzyme F420-1:γ-L-glutamate ligase (EC 6.3.2.34, F420:gamma-glutamyl ligase, CofE-AF, MJ0768, CofE) is an enzyme with systematic name L-glutamate:coenzyme F420-1 ligase (GDP-forming).[1][2] This enzyme catalyses the following chemical reaction
| Coenzyme F420-1:γ-L-glutamate ligase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC no. | 6.3.2.34 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| |||||||||
- GTP + coenzyme F420-1 + L-glutamate GDP + phosphate + coenzyme γ-F420-2
In some instances, this enzyme is fused with a coenzyme F420-0:L-glutamate ligase domain, so that the coenzyme F420-1 produced by the latter is quickly ligated to coenzyme F420-2. This enzyme is also able to serially add additional γ-glutamyl groups to F420-2, producing F420-3 and so on.[3]
References
edit- ^ Li H, Graupner M, Xu H, White RH (August 2003). "CofE catalyzes the addition of two glutamates to F420-0 in F420 coenzyme biosynthesis in Methanococcus jannaschii". Biochemistry. 42 (32): 9771–9778. doi:10.1021/bi034779b. PMID 12911320.
- ^ Nocek B, Evdokimova E, Proudfoot M, Kudritska M, Grochowski LL, White RH, et al. (September 2007). "Structure of an amide bond forming F(420):gamma-glutamyl ligase from Archaeoglobus fulgidus -- a member of a new family of non-ribosomal peptide synthases". Journal of Molecular Biology. 372 (2): 456–469. doi:10.1016/j.jmb.2007.06.063. PMC 2678844. PMID 17669425.
- ^ "Rhea- Annotated reactions database". www.rhea-db.org.
Choi KP, Bair TB, Bae YM, Daniels L (December 2001). "Use of transposon Tn5367 mutagenesis and a nitroimidazopyran-based selection system to demonstrate a requirement for fbiA and fbiB in coenzyme F(420) biosynthesis by Mycobacterium bovis BCG". Journal of Bacteriology. 183 (24): 7058–7066. doi:10.1128/JB.183.24.7058-7066.2001. PMC 95553. PMID 11717263.
External links
edit- Coenzyme+F420-1:gamma-L-glutamate+ligase at the U.S. National Library of Medicine Medical Subject Headings (MeSH)
