DBU may refer to:
- 1,8-Diazabicyclo[5.4.0]undec-7-ene, an organic chemical, amidine base
- Desh Bhagat University, Mandi Gobindgarh, Punjab, India
- Dallas Baptist University, a Christian liberal arts university in Dallas, Texas
- Dansk Boldspil-Union, the Danish Football Association
- dBu, abbreviation of decibel μV, a logarithmic measurement of voltage relative to 1 μV
- DBU, ISO 639-3 code for the Bondum Dogon language
- Duluth Business University, a private university in Duluth, Minnesota