Wikipedia

Do-it-yourself biology

(Redirected from DIY biology)
"DIYbio" redirects here. For the organization, see DIYbio (organization).
This article is about real-life citizen science and advocacy movement. For dystopian cyberpunk and science fiction subgenre, see Biopunk.

Do-it-yourself biology (DIY biology, DIY bio) is a biotechnological social movement in which individuals, communities, and small organizations study biology and life science using the same methods as traditional research institutions. DIY biology is primarily undertaken by individuals with limited research training from academia or corporations, who then mentor and oversee other DIY biologists with little or no formal training.[1] This may be done as a hobby, as a not-for-profit endeavor for community learning and open-science innovation, or for profit, to start a business.

Preparation of a biohacking kit for a biology workshop in Popular Education in a café in Rennes in 2020

Other terms are also associated with the do-it-yourself biology community. The terms biohacking and wetware hacking emphasize the connection to hacker culture and the hacker ethic.[2] The term hacker is used in the original sense of finding new and clever ways to do things. The term biohacking is also used by the grinder body modification community, which is considered related but distinct from the do-it-yourself biology movement.[3] The term biopunk emphasizes the techno-progressive, political, and artistic elements of the movement.

History

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The term "biohacking" as well as the concept of do-it-yourself biology has been known as early as 1988.[4][5][6]

Biohacking entered the San Francisco programmer and maker communities as early as 2005, through simple demonstrations of basic experiments. As DIYbio experiments became the focus of SuperHappyDevHouse hackers, the hobby gained additional momentum.

In 2005 Rob Carlson wrote in an article in Wired: "The era of garage biology is upon us. Want to participate? Take a moment to buy yourself a lab on eBay."[7] He then set up a garage lab the same year, working on a project he had previously worked at the Molecular Sciences Institute in Berkeley, California.[8]

In 2008, the DIYbio organization was founded by Jason Bobe and Mackenzie Cowell and its first meeting held.[9]

In 2010, Genspace opened the first community biology lab,[10] Ten months later it was followed by BioCurious,[11] and Victoria Makerspace. Many other labs and organizations followed, including but not limited to Counter Culture Labs in Oakland, CA, Baltimore Underground Science Space in Baltimore, MD, TheLab in Los Angeles, CA and Denver Biolabs in Denver, CO.

It has been estimated that in 2014 there have been 50 DIY biology labs around the world.[12]: 119 

In 2016, the first conference to focus specifically on biohacking was announced to take place in September in Oakland, CA.[13]

Aspects

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The DIYbio movement seeks to revise the notion that one must be an academic with an advanced degree to make any significant contribution to the biology community. It allows large numbers of small organizations and individuals to participate in research and development, with spreading knowledge a higher priority than turning profits.[14] In recent years, there are various DIY ways to live healthy and many of them also focuses on different simple ways to biohack mind,[15] body, metabolism[16][better source needed][17][18] and sleep.[19]

The motivations for DIY biology include (but aren't limited to) lowered costs, entertainment, medicine, biohacking, life extension, and education. Recent work combining open-source hardware of microcontrollers like the Arduino and RepRap 3-D printers, very low-cost scientific instruments have been developed.[20]

Community laboratory space

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Many organizations maintain a laboratory akin to a wet-lab makerspace, providing equipment and supplies for members. Many organizations also run classes and provide training. For a fee (usually between $50 and $100), members can join some spaces and do experiments on their own.[21][22][23]

Open source equipment

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The DIY biology movement attempts to make available the tools and resources necessary for anyone, including non-professionals, to conduct biological engineering. One of the first pieces of open source laboratory equipment developed was the Dremelfuge by Irish biohacker Cathal Garvey, which uses a 3D printed tube holder attached to a Dremel rotary tool to spin tubes at high speeds, replacing often expensive centrifuges.[24] Many other devices like PCR machines have been recreated extensively.[25][26][27] In recent times, more complex devices have been created such as the OpenDrop digital microfluidics platform[28] and the DIY NanoDrop[29] both developed by GaudiLabs. Opentrons makes open-source software, affordable lab robots, and got its start as a DIY biology collaboration at Genspace.[30] Incuvers makes telemetric chambers for cellular research that are affordable and allow for complete customizability of their environments. OpenCell, a London based biotech lab provider hosts regular biohackathons to help encourage more opensource development.[31]

Advocacy

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Most advocacy in biohacking is about the safety, accessibility and future legality of experimentation. Todd Kuiken of the Woodrow Wilson Center proposes that through safety and self-governance, DIY biologists won't be in need of regulation.[32] Josiah Zayner has proposed that safety is inherent in biohacking and that accessibility should be the foremost concern as there is large underrepresentation of social and ethnic minorities in biohacking.[33]

Research topics

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Many biohacking projects revolve around the modification of life and molecular and genetic engineering.[34]

Genetic engineering

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Genetic Engineers are a subculture of biohackers as one of the most accessible forms of biohacking is through engineering microorganisms or plants. Experiments can range from using plasmids to fluorescent bacteria, controlling gene expression using light in bacteria,[35] even using CRISPR to engineer the genome of bacteria or yeast.[36]

Medicine

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Main article: DIY medicine

Restricted access to medical care and medicine has pushed biohackers to start experimenting in medically related fields. The Open Insulin project aims to make the recombinant protein insulin more accessible by creating an open source protocol for expression and purification.[37] Other experiments that have involved medical treatments include a whole body microbiome transplant[38] and the creation of open source artificial pancreases[39] for diabetics, such as OpenAPS, Loop[40] and AndroidAPS.[41]

Implants

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Grinders are a subculture of biohackers that focus on implanting technology[42] or introducing chemicals[43] into the body to enhance or change their bodies' functionality.

Some biohackers can now sense which direction they face using a magnetic implant that vibrates against the skin.[44]

Art

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In 2000, controversial and self-described "transgenic artist" Eduardo Kac appropriated standard laboratory work by biotechnology and genetics researchers in order to both utilize and critique such scientific techniques. In the only putative work of transgenic art by Kac, the artist claimed to have collaborated with a French laboratory (belonging to the Institut National de la Recherche Agronomique) to procure a green-fluorescent rabbit: a rabbit implanted with a green fluorescent protein gene from a type of jellyfish [Aequorea victoria] in order for the rabbit to fluoresce green under ultraviolet light. The claimed work came to be known as the "GFP bunny", and which Kac called Alba. This claim by Kac has been disputed by the scientists at the lab who noted that they had performed exactly the same experiment (i.e., the insertion of the jellyfish GFP protein-coding gene) on numerous other animals (cats, dogs, etc.) previously and did not create Alba (known to the researchers only as "Rabbit Number 5256") under the direction of Kac. The laboratory consequently kept possession of the transgenic rabbit which it had created and funded and the "transgenic art" was never exhibited at the Digital Avignon festival [2000] as intended. Kac—claiming that his rabbit was the first GFP bunny created in the name of Art—used this dispute to popularize the issue as one of disguised censorship by launching a "Free Alba" campaign. A doctored photo of the artist holding a day-glow-green tinted rabbit appears on his website.[45] The members of the Critical Art Ensemble have written books and staged multimedia performance interventions around this issue, including The Flesh Machine (focusing on in vitro fertilisation, surveillance of the body, and liberal eugenics) and Cult of the New Eve (In order to analyze how, in their words, "Science is the institution of authority regarding the production of knowledge, and tends to replace this particular social function of conventional Christianity in the west").[46]

Heather Dewey-Hagborg is an information artist and biohacker who uses genomic DNA left behind by people as a starting point for creating lifelike, computer-generated, 3-D portraits.[47][48]

Criticism and concerns

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Biohacking experiences many of the same criticisms as synthetic biology and genetic engineering already receive, plus other concerns relating to the distributed and non-institutional nature of the work, involving potential hazards with lack of oversight by professionals or governments. Concerns about biohackers creating pathogens in unmonitored garage laboratories led the Federal Bureau of Investigation (FBI) to begin sending its representatives to DIYbio conferences in 2009.[8] The arrest and prosecution of some members for their work with harmless microbes, such as artivist Steve Kurtz, has been denounced as political repression by critics who argue the U.S. government has used post-9/11 anti-terrorism powers to intimidate artists and others who use their art to criticize society.[49]

Existing regulations are not specific to this field, so that the possibility of pathogenic organisms being created and released unintentionally or intentionally by biohackers has become a matter of concern, for example, in the spirit of the re-creation of the 1917 flu virus by Armed Forces Institute of Pathology researchers in 2005.[50] In the US the FBI Weapons of Mass Destruction Directorate has worked with the American Association for the Advancement of Science's National Science Advisory Board for Biosecurity to convene a series of meetings to discuss biosecurity, which have included discussions of amateur biologists and ways to manage the risks to society it poses.[51][52]: 8.16  At the National Institutes of Health, National Science Advisory Board for Biosecurity[53] leads efforts to educate the public on "dual use research of concern", for example with websites like "Science Safety Security".[54] In 2011, DIYbio organized conferences to attempt to create codes of ethics for biohackers.[55]

A 2007 ETC Group report warns that the danger of this development is not just bioterror, but "bio-error".[56]

While detractors argue that do-it-yourself biologists need some sort of supervision, enthusiasts argue that uniform supervision is impossible and the best way to prevent accidents or malevolence is to encourage a culture of transparency, where, in essence, do-it-yourself biologists would be peer reviewed by other biohackers.[57] DIYbio argues that fear of potential hazards should be met with increased research and education rather than closing the door on the profound positive impacts that distributed biological technology will have on human health, the environment, and the standard of living around the world.[58] Due to the lack of precedent regarding such a business model, the DIYbio founders see this as an opportunity to be innovators in regulatory and safety policy.[9]

Groups and organizations

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See also

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References

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  1. ^ "Do-it-yourself biology shows safety risks of an open innovation movement". Brookings. Retrieved 2024-03-15.
  2. ^ Hicks, Jennifer (2014-03-15). "The Biohacking Hobbyist". Forbes. Retrieved 2016-06-19.
  3. ^ Michels, Spencer (2014-09-23). "What is biohacking and why should we care?". PBS NewsHour. Retrieved 2015-10-30.
  4. ^ Katz, Sylvan (6 January 1990). "Forum: Roses are black, violets are green – The emergence of amateur genetic engineers". New Scientist. Retrieved 2015-10-25.
  5. ^ Katz, J. S. (1990). "That which is not forbidden is Mandatory". Education. 4 (1). ISSN 0955-6621.
  6. ^ Schrage, Michael (1988-01-31). "Playing God in your basement". The Washington Post. ISSN 0190-8286. Retrieved 2015-10-25.
  7. ^ Carlson, Rob (May 2005). "Splice It Yourself: Who needs a geneticist? Build your own lab". Wired.
  8. ^ a b Ledford, Heidi (2010). "Garage : Life hackers". Nature. 467 (7316): 650–2. doi:10.1038/467650a. PMID 20930820.
  9. ^ a b "PBS News Hour". YouTube. 31 Dec 2008. Archived from the original on 2021-12-20.
  10. ^ Mosher, Dave (2010-12-16). "DIY Biotech Hacker Space Opens in NYC". Wired. Retrieved 2017-07-25.
  11. ^ "BioCurious Officially Opens — Test DNA, Build Equipment, Find a Co-founder and More…". Make Magazine. 2011-10-14. Retrieved 2017-07-25.
  12. ^ Dariusz Jemielniak; Aleksandra Przegalinska (18 February 2020). Collaborative Society. MIT Press. ISBN 978-0-262-35645-9.
  13. ^ "Biohack the Planet Conference". Biohack the Planet. Retrieved 2016-06-19.
  14. ^ "Rob Carlson on synthetic biology". The Economist. Archived from the original on 2011-10-09.
  15. ^ Wu, Chen-Yi; Hu, Hsiao-Yun; Chow, Lok-Hi; et al. (22 June 2015). "The Effects of Anti-Dementia and Nootropic Treatments on the Mortality of Patients with Dementia: A Population-Based Cohort Study in Taiwan". PLOS ONE. 10 (6): e0130993. Bibcode:2015PLoSO..1030993W. doi:10.1371/journal.pone.0130993. PMC 4476616. PMID 26098910.
  16. ^ Singh, Honey (2 February 2020). "How to Biohack Your Metabolism?". Do BioHacking.
  17. ^ Heindel, Jerrold J.; Blumberg, Bruce; Cave, Mathew; et al. (March 2017). "Metabolism disrupting chemicals and metabolic disorders". Reproductive Toxicology. 68: 3–33. Bibcode:2017RepTx..68....3H. doi:10.1016/j.reprotox.2016.10.001. PMC 5365353. PMID 27760374.
  18. ^ Prigent, Sylvain; Frioux, Clémence; Dittami, Simon M.; Thiele, Sven; Larhlimi, Abdelhalim; Collet, Guillaume; Gutknecht, Fabien; Got, Jeanne; Eveillard, Damien; Bourdon, Jérémie; Plewniak, Frédéric; Tonon, Thierry; Siegel, Anne (27 January 2017). "Meneco, a Topology-Based Gap-Filling Tool Applicable to Degraded Genome-Wide Metabolic Networks". PLOS Computational Biology. 13 (1): e1005276. Bibcode:2017PLSCB..13E5276P. doi:10.1371/journal.pcbi.1005276. PMC 5302834. PMID 28129330.
  19. ^ Anderson, Jason R.; Hagerdorn, Payton L.; Gunstad, John; Spitznagel, Mary Beth (July 2018). "Using coffee to compensate for poor sleep: Impact on vigilance and implications for workplace performance". Applied Ergonomics. 70: 142–147. doi:10.1016/j.apergo.2018.02.026. PMID 29866304. S2CID 46929880.
  20. ^ Pearce, Joshua M. (14 September 2012). "Building Research Equipment with Free, Open-Source Hardware". Science. 337 (6100): 1303–1304. Bibcode:2012Sci...337.1303P. doi:10.1126/science.1228183. PMID 22984059. S2CID 44722829.
  21. ^ "BUGSS Membership". BUGSS. Archived from the original on 2017-08-25. Retrieved 2016-06-17.
  22. ^ "Biocurious Membership". Biocurious. Archived from the original on 2017-05-10. Retrieved 2016-06-17.
  23. ^ "Counter Culture Labs Membership". Counter Culture Labs. Retrieved 2016-06-17.
  24. ^ "DremelFuge – A One-Piece Centrifuge for Rotary Tools". Thingiverse. 2009-12-23. Retrieved 2016-06-17.
  25. ^ Jankowski, Tito (2011-07-06). "DNA is now DIY: OpenPCR ships worldwide". Make.
  26. ^ "Pocket PCR for pennies". LavaAmp. Archived from the original on 2015-11-06. Retrieved 2015-10-26.
  27. ^ "Coffee Cup – PCR Thermocycler costing under 350$". Instructables. 2009-06-13. Retrieved 2016-06-17.
  28. ^ "OpenDrop". OpenDrop. Retrieved 2016-06-17.
  29. ^ "DIY NanoDrop". DIY NanoDrop. Hackteria. Retrieved 2016-06-17.
  30. ^ Landoni, Boris (11 July 2014). "Interview to Open Trons | Open Electronics". Open Source Electronics. Retrieved 1 November 2016.
  31. ^ "Open Cell is hosting monthly biohackathons to develop equipment, software and products to make better and more affordable biolaboratories". OpenCell.bio. OpenCell. Retrieved 2019-08-17.
  32. ^ Todd Kuiken (2016-03-09). "Governance: Learn from DIY biologists". Nature Magazine. 531 (7593): 167–168. Bibcode:2016Natur.531..167K. doi:10.1038/531167a. PMID 26961642.
  33. ^ Yin, Steph (2016-05-03). "Is DIY Kitchen CRISPR A Class Issue?". Popular Science. Retrieved 2016-06-19.
  34. ^ Hicks, Jennifer (2014-03-15). "The Biohacking Hobbyist". Forbes. Retrieved 2016-06-19.
  35. ^ "Biocurious Meetup". Meetup. 2016-06-25. Retrieved 2016-06-17.
  36. ^ Krieger, Lisa M. (2016-01-11). "Bay Area biologist's gene-editing kit lets do-it-yourselfers play God at the kitchen table". San Jose Mercury News. Retrieved 2016-06-17.
  37. ^ Ossola, Alexandra (2015-11-18). "These Biohackers Are Creating Open-Source Insulin". Popular Science. Retrieved 2016-06-17.
  38. ^ Duhaime-Ross, Arielle (2016-05-04). "A Bitter Pill". The Verge.
  39. ^ Linebaugh, Kate (2016-05-09). "Tech-savvy Families use homebuilt diabetes device". Wall Street Journal.
  40. ^ "LoopDocs". loopkit.github.io. Retrieved 2022-07-25.
  41. ^ "Welcome to the AndroidAPS documentation — AndroidAPS 3.0 documentation". androidaps.readthedocs.io. Retrieved 2022-07-25.
  42. ^ Neifer, Anna (2015-11-09). "Biohackers are implanting LEDs under their skin". Motherboard.
  43. ^ Dvorsky, George (2016-03-27). "This Biohacker Used Eyedrops To Give Himself Temporary Night Vision". Gizmodo.
  44. ^ Thaddeus-Johns, Josie (2017-01-06). "Meet the first humans to sense where north is". The Guardian. Retrieved 2018-06-02.
  45. ^ Dickey, Christopher (April 2001). "I Love My Glow Bunny". {{cite magazine}}: Cite magazine requires |magazine= (help)
  46. ^ "Critical Art Ensemble". critical-art.net. Archived from the original on 2015-11-13. Retrieved 2015-11-20.
  47. ^ Jenkins, Mark (2013-09-18). "A 'Cyber' exhibit as timely as the news". Washington Post. p. E18.
  48. ^ Krulwich, Robert (2013-06-28). "Artist plays detective: Can I reconstruct a face from a piece of hair?". NPR. Retrieved 7 August 2014.
  49. ^ The Associated Press (October 29, 2007). "Scientist pleads guilty to mailing bacteria for 'bio-art'". First Amendment Center. Archived from the original on 2009-02-10.
  50. ^ "The 1918 flu virus is resurrected". Nature. 437 (7060): 794–5. October 2005. Bibcode:2005Natur.437..794.. doi:10.1038/437794a. PMC 7095040. PMID 16208326.
  51. ^ Zimmer, Carl (March 5, 2012). "Amateurs Are New Fear in Creating Mutant Virus". The New York Times.
  52. ^ Prepared by the American Association for the Advancement of Science in conjunction with the Association of American Universities, Association of Public and Land-grant Universities, and the Federal Bureau of Investigation Bridging Science and Security for Biological Research: A Discussion about Dual Use Review and Oversight at Research Institutions Report of a Meeting September 13–14, 2012 Archived 2012-10-28 at the Wayback Machine
  53. ^ "NSABB Official Website". Archived from the original on 2015-11-17. Retrieved 2015-10-26.
  54. ^ Science Safety Security official website
  55. ^ "The role of codes of conduct in the amateur biology community". Retrieved 4 February 2014.
  56. ^ ETC Group (January 2007). "Extreme Genetic Engineering: An Introduction to Synthetic Biology" (PDF). Retrieved 2012-09-28.
  57. ^ Boustead, Greg (December 11, 2008). "The Biohacking Hobbyist". Seed Magazine. Archived from the original on 2019-10-06.{{cite journal}}: CS1 maint: unfit URL (link)
  58. ^ "DIYbio/FAQ".
 
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