do Amaral is a surname. Notable people with the surname include:
- Amarildo Souza do Amaral (born 1964), Brazilian football player
- Carlos do Amaral Freire, Brazilian scholar, linguist and translator
- Carlos Rafael do Amaral (born 1983), Brazilian football player
- Crispim do Amaral (1858–1911), Brazilian artist who painted the curtain at the Amazon Theatre
- Francisco Keil do Amaral (1910–1975), Portuguese architect, composer, painter and photographer
- Francisco Xavier do Amaral (1937–2012), East Timorese politician
- Maria do Carmo Estanislau do Amaral (born 1959), Brazilian botanist
- Tarsila do Amaral (1886–1973), Brazilian modernist artist