Germacrene A hydroxylase (EC 1.14.13.123) is an enzyme with systematic name (+)-germacrene-A,NADPH:oxygen oxidoreductase (12-hydroxylating).[1][2] This enzyme catalyses the following chemical reaction
| Germacrene A hydroxylase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC no. | 1.14.13.123 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| |||||||||
- (+)-germacrene A + NADPH + H+ + O2 germacra-1(10),4,11(13)-trien-12-ol + NADP+ + H2O
Germacrene A hydroxylase is a heme-thiolate protein (P-450).
References
edit- ^ de Kraker JW, Franssen MC, Dalm MC, de Groot A, Bouwmeester HJ (April 2001). "Biosynthesis of germacrene A carboxylic acid in chicory roots. Demonstration of a cytochrome P450 (+)-germacrene a hydroxylase and NADP+-dependent sesquiterpenoid dehydrogenase(s) involved in sesquiterpene lactone biosynthesis". Plant Physiology. 125 (4): 1930–40. doi:10.1104/pp.125.4.1930. PMC 88848. PMID 11299372.
- ^ Nguyen DT, Göpfert JC, Ikezawa N, Macnevin G, Kathiresan M, Conrad J, Spring O, Ro DK (May 2010). "Biochemical conservation and evolution of germacrene A oxidase in asteraceae". The Journal of Biological Chemistry. 285 (22): 16588–98. doi:10.1074/jbc.M110.111757. PMC 2878029. PMID 20351109.
External links
edit- Germacrene+A+hydroxylase at the U.S. National Library of Medicine Medical Subject Headings (MeSH)
