Lipid IVA 3-deoxy-D-manno-octulosonic acid transferase (EC 2.4.99.12, KDO transferase, waaA (gene), kdtA (gene), 3-deoxy-D-manno-oct-2-ulosonic acid transferase, 3-deoxy-manno-octulosonic acid transferase, lipid IVA KDO transferase) is an enzyme with systematic name CMP-3-deoxy-D-manno-oct-2-ulosonate:lipid IVA 3-deoxy-D-manno-oct-2-ulosonate transferase.[1][2][3][4] This enzyme catalyses the following chemical reaction
| Lipid IVA 3-deoxy-D-manno-octulosonic acid transferase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC no. | 2.4.99.12 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| |||||||||
- lipid IVA + CMP-alpha-Kdo alpha-Kdo-(2->6)-lipid IVA + CMP
The bifunctional enzyme from Escherichia coli transfers two 3-deoxy-D-man no-oct-2-resonate residues to lipid IVA.
See also
edit- EC 2.4.99.13, (KDO)-lipid IVA 3-deoxy-D-manno-octulosonic acid transferase
References
edit- ^ Belunis CJ, Raetz CR (May 1992). "Biosynthesis of endotoxins. Purification and catalytic properties of 3-deoxy-D-manno-octulosonic acid transferase from Escherichia coli". The Journal of Biological Chemistry. 267 (14): 9988–97. doi:10.1016/S0021-9258(19)50189-2. PMID 1577828.
- ^ Mamat U, Schmidt H, Munoz E, Lindner B, Fukase K, Hanuszkiewicz A, Wu J, Meredith TC, Woodard RW, Hilgenfeld R, Mesters JR, Holst O (August 2009). "WaaA of the hyperthermophilic bacterium Aquifex aeolicus is a monofunctional 3-deoxy-D-manno-oct-2-ulosonic acid transferase involved in lipopolysaccharide biosynthesis". The Journal of Biological Chemistry. 284 (33): 22248–62. doi:10.1074/jbc.M109.033308. PMC 2755949. PMID 19546212.
- ^ White KA, Kaltashov IA, Cotter RJ, Raetz CR (June 1997). "A mono-functional 3-deoxy-D-manno-octulosonic acid (Kdo) transferase and a Kdo kinase in extracts of Haemophilus influenzae". The Journal of Biological Chemistry. 272 (26): 16555–63. doi:10.1074/jbc.272.26.16555. PMID 9195966.
- ^ Löbau S, Mamat U, Brabetz W, Brade H (November 1995). "Molecular cloning, sequence analysis, and functional characterization of the lipopolysaccharide biosynthetic gene kdtA encoding 3-deoxy-alpha-D-man no-octulosonic acid transferase of Chlamydia pneumoniae strain TW-183". Molecular Microbiology. 18 (3): 391–9. doi:10.1111/j.1365-2958.1995.mmi_18030391.x. PMID 8748024.
External links
edit- Lipid+IVA+3-deoxy-D-manno-octulosonic+acid+transferase at the U.S. National Library of Medicine Medical Subject Headings (MeSH)
