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Summary
| Description |
English: A scheme of using PACE to evolve orthogonal aminoacyl-tRNA synthetases. Without synthetase activity, T7 RNAP cannot be fully translated due to the presence of an Amber stop codon. Upon introduction of a functioning synthetase for the Amber codon tRNA, this Amber stop codon now encodes a non-canonical amino acid, allowing for the transcription of the full T7 RNAP and hence allowing gIII transcription. |
| Date | |
| Source | Own work |
| Author | Alexjli |
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| Date/Time | Thumbnail | Dimensions | User | Comment | |
|---|---|---|---|---|---|
| current | 03:48, 13 May 2020 | | 1,863 × 418 (41 KB) | Alexjli | Cross-wiki upload from en.wikipedia.org |
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