Comparison of dimension-unlimited fluorescence imaging cycler microscopy (ICM) and standard three-parameter fluorescence microscopy
DescriptionComparison of dimension-unlimited epifluorescence imaging cycler microscopy (ICM) and standard three-parameter fluorescence microscopy..jpg
English: (a) Dermoepithelial junction (human skin tissue section) measured by three-parameter fluorescence microscopy. (b) ICM, same location as in (a) resolves the basal lamina densa (LD) as a giant stoichiometrically controlled supermolecule and discerns the three basal lamina layers (lamina lucida, LL; lamina densa, LD, and lamina fibroreticularis, LF) as indicated by comparison with a transmission electron micrograph (c). (d) Profiles of 100 distinct biomolecules co-mapped by ICM. (e) Three-dimensional network of supermolecules (different colours, measured by ICM) on the cell surface of a single human blood T-lymphocyte[1][2]. (f) More than 7,000 different protein clusters in a human liver cell measured by ICM[3].
↑Friedenberger, Manuela; Bode, Marcus; Krusche, Andreas; Schubert, Walter (September 2007). "Fluorescence detection of protein clusters in individual cells and tissue sections by using toponome imaging system: sample preparation and measuring procedures". Nature Protocols2 (9): 2285–2294. DOI:10.1038/nprot.2007.320.
↑Schubert, W (2014). "Systematic, spatial imaging of large multimolecular assemblies and the emerging principles of supramolecular order in biological systems.". Journal of Molecular Recognition27 (1): 3-18. DOI:10.1002/jmr.2326.
↑Schubert, Walter; Bonnekoh, Bernd; Pommer, Ansgar J; Philipsen, Lars; Böckelmann, Raik; Malykh, Yanina; Gollnick, Harald; Friedenberger, Manuela; Bode, Marcus; Dress, Andreas W M (1 October 2006). "Analyzing proteome topology and function by automated multidimensional fluorescence microscopy". Nature Biotechnology24 (10): 1270–1278. DOI:10.1038/nbt1250.
Licensing
I, the copyright holder of this work, hereby publish it under the following license:
to share – to copy, distribute and transmit the work
to remix – to adapt the work
Under the following conditions:
attribution – You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.
share alike – If you remix, transform, or build upon the material, you must distribute your contributions under the same or compatible license as the original.