DescriptionImmobilization Strategies for Target Identification.png
English: 1A. A small molecule is derivatized for immobilization on a solid support, such as a magnetic bead or affinity column. Protein targets bind to the small molecule and can be identified after elution.
1B. A small molecule is attached to an enrichment handle. The enrichment handle is then used to pull down the small molecule-protein complex.
1C. A small molecule molecule is functionalized with a cross-linking group and an enrichment handle. After a target binds the small molecule, the cross-linking group can be activated, often by light, forming a covalent bond between target and small molecule. The enrichment handle is then used to pull down the small molecule-protein complex.
2. Schematic of an immobilization based proteomics workflow. Immobilized targets are digested and analyzed using LC-MS/MS.
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