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Summary
| Description |
English: Mitochondrial outer membrane proteins are identified via proximity labeling. APEX2 is an engineered enzyme that oxidizes biotin-phenol to biotin-phenol free radical using hydrogen peroxide. In this proximity labeling experiment to identify mitochondrial outer membrane proteins, APEX2 is fused to a known mitochondrial outer membrane protein in live cells. Biotin-phenol and hydrogen peroxide are then added sequentially. APEX2 oxidation causes biotin-phenol to react with nearby proteins. The proteins are pulled down with streptavidin beads for subsequent identification by mass spectrometry proteomics. Figure generated with Assets used under terms of the BioRender Basic License. Created with [1]. |
| Date | 5/4/20 |
| Source | Own work |
| Author | Kbufdbqjubmjtn |
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| Date/Time | Thumbnail | Dimensions | User | Comment | |
|---|---|---|---|---|---|
| current | 02:06, 5 May 2020 | | 863 × 863 (183 KB) | Kbufdbqjubmjtn | Uploaded own work with UploadWizard |
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