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Summary

Description	English: Localisation microscopy: SPDMphymod images of Tobacco mosaic virus (TMV) particles with coated proteins labeled with the fluorophore Atto448. B: after Gaussian blurring corresponding to the individual localization accuracy D: same data, but distribution of the individual molecule position without Gaussain blurring Virus Super Resolution Microscopy/Optical nanoscopy: A further example for the use of localization microscopy/SPDM is an analysis of Tobacco mosaic virus (TMV) particles. TMV particles are not only important in plant research but are also an example for a class of most promising biomolecule complexes exhibiting a high potential for nanotechnology applications as virus-derived biotemplates for nanostructured hybrid materials and building-blocks in analytical, technical & therapeutic applications. Collaboration of the former Christoph Cremer lab, emeritus at Heidelberg university ^[1], ^[2] & the Wege lab (Universities of Stuttgart). Reference: Superresolution imaging of biological nanostructures by spectral precision distance microscopy (2011): Cremer, R. Kaufmann, M. Gunkel, S. Pres, Y. Weiland, P- Müller, T. Ruckelshausen, P. Lemmer, F. Geiger, S. Degenhard, C. Wege, N- A. W. Lemmermann, R. Holtappels, H. Strickfaden, M. Hausmann , Biotechnology 6: 1037–1051 See also [1] Prof. Christina Wege [2] Prof. Christoph Cremer
Date	11 September 2012, 12:08:39
Source	Own work
Author	Andy Nestl

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Gallery

Super Resolution Microscopy - Localisation Microscopy
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Single YFP molecule detection in a human cancer cell. Typical distance measurements 15 nm
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Label-free Localisation Microscopy SPDM - Super Resolution Microscopy reveals prior undetebable intracellular structures
Investigation of human eye tissue, affected by macular degeneration AMD
Virus Super Resolution Microscopy SPDM Cremer/Wege labs