InaD-like protein

InaD-like protein is a protein that in humans is encoded by the PATJ gene.

PATJ
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 1VF6, 2D92, 2DAZ, 2DB5, 2DLU, 2DM8, 2DMZ, 2EHR, 4Q2N
Identifiers
Aliases	PATJ, Cipp, InaD-like, hINADL, INADL, crumbs cell polarity complex component, PATJ crumbs cell polarity complex component
External IDs	OMIM: 603199; MGI: 1277960; HomoloGene: 72199; GeneCards: PATJ; OMA:PATJ - orthologs
Gene location (Human) Chr. Chromosome 1 (human) Band 1p31.3 Start 61,742,477 bp End 62,178,675 bp
Gene location (Mouse) Chr. Chromosome 4 (mouse) Band 4|4 C6 Start 98,284,022 bp End 98,607,840 bp
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in right ventricle renal medulla pons cerebellar cortex cerebellar hemisphere Achilles tendon right hemisphere of cerebellum Skeletal muscle tissue of rectus abdominis gingival epithelium cerebellar vermis Top expressed in cerebellar vermis lateral geniculate nucleus lobe of cerebellum medial dorsal nucleus medial geniculate nucleus motor neuron facial motor nucleus anterior horn of spinal cord inferior colliculi deep cerebellar nuclei More reference expression data BioGPS More reference expression data
Gene ontology Molecular function protein binding Cellular component cytoplasm perinuclear region of cytoplasm plasma membrane apical part of cell extracellular exosome apical plasma membrane membrane bicellular tight junction centrosome microtubule organizing center cytosol cell junction protein-containing complex Biological process intracellular signal transduction bicellular tight junction assembly Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 10207 12695 Ensembl ENSG00000132849 ENSMUSG00000061859 UniProt Q8NI35 Q63ZW7 RefSeq (mRNA) NM_005799 NM_176877 NM_176878 NM_001350145 NM_001005784 NM_001005787 NM_007704 NM_172696 NM_001355177 RefSeq (protein) NP_795352 NP_001337074 NP_001005784 NP_001005787 NP_031730 NP_766284 NP_001342106 Location (UCSC) Chr 1: 61.74 – 62.18 Mb Chr 4: 98.28 – 98.61 Mb PubMed search
Wikidata
View/Edit Human View/Edit Mouse

Function

This gene encodes a protein with multiple PDZ domains. PDZ domains mediate protein-protein interactions, and proteins with multiple PDZ domains often organize multimeric complexes at the plasma membrane. This protein localizes to tight junctions and to the apical membrane of epithelial cells. A similar protein in Drosophila is a scaffolding protein which tethers several members of a multimeric signaling complex in photoreceptors.

Interactions

INADL has been shown to interact with MPP5.

Further reading

• Fanning AS, Anderson JM (1999). "PDZ domains: fundamental building blocks in the organization of protein complexes at the plasma membrane". J. Clin. Invest. 103 (6): 767–72. doi:10.1172/JCI6509. PMC 408156. PMID 10079096.
• Lennon G, Auffray C, Polymeropoulos M, Soares MB (1996). "The I.M.A.G.E. Consortium: an integrated molecular analysis of genomes and their expression". Genomics. 33 (1): 151–2. doi:10.1006/geno.1996.0177. PMID 8617505.
• Kurschner C, Mermelstein PG, Holden WT, Surmeier DJ (1998). "CIPP, a novel multivalent PDZ domain protein, selectively interacts with Kir4.0 family members, NMDA receptor subunits, neurexins, and neuroligins". Mol. Cell. Neurosci. 11 (3): 161–72. doi:10.1006/mcne.1998.0679. PMID 9647694. S2CID 36534759.
• Vaccaro P, Brannetti B, Montecchi-Palazzi L, Philipp S, Helmer Citterich M, Cesareni G, Dente L (2001). "Distinct binding specificity of the multiple PDZ domains of INADL, a human protein with homology to INAD from Drosophila melanogaster". J. Biol. Chem. 276 (45): 42122–30. doi:10.1074/jbc.M104208200. PMID 11509564.
• Anzai N, Deval E, Schaefer L, Friend V, Lazdunski M, Lingueglia E (2002). "The multivalent PDZ domain-containing protein CIPP is a partner of acid-sensing ion channel 3 in sensory neurons". J. Biol. Chem. 277 (19): 16655–61. doi:10.1074/jbc.M201087200. PMID 11872753.
• Roh MH, Makarova O, Liu CJ, Shin K, Lee S, Laurinec S, Goyal M, Wiggins R, Margolis B (2002). "The Maguk protein, Pals1, functions as an adapter, linking mammalian homologues of Crumbs and Discs Lost". J. Cell Biol. 157 (1): 161–72. doi:10.1083/jcb.200109010. PMC 2173254. PMID 11927608.
• Lemmers C, Médina E, Delgrossi MH, Michel D, Arsanto JP, Le Bivic A (2002). "hINADl/PATJ, a homolog of discs lost, interacts with crumbs and localizes to tight junctions in human epithelial cells". J. Biol. Chem. 277 (28): 25408–15. doi:10.1074/jbc.M202196200. PMID 11964389.
• Roh MH, Liu CJ, Laurinec S, Margolis B (2002). "The carboxyl terminus of zona occludens-3 binds and recruits a mammalian homologue of discs lost to tight junctions". J. Biol. Chem. 277 (30): 27501–9. doi:10.1074/jbc.M201177200. PMID 12021270.
• Makarova O, Roh MH, Liu CJ, Laurinec S, Margolis B (2003). "Mammalian Crumbs3 is a small transmembrane protein linked to protein associated with Lin-7 (Pals1)". Gene. 302 (1–2): 21–9. doi:10.1016/S0378111902010843. PMID 12527193.
• Roh MH, Fan S, Liu CJ, Margolis B (2004). "The Crumbs3-Pals1 complex participates in the establishment of polarity in mammalian epithelial cells". J. Cell Sci. 116 (Pt 14): 2895–906. doi:10.1242/jcs.00500. PMID 12771187. S2CID 4482921.
• Li Y, Karnak D, Demeler B, Margolis B, Lavie A (2005). "Structural basis for L27 domain-mediated assembly of signaling and cell polarity complexes". EMBO J. 23 (14): 2723–33. doi:10.1038/sj.emboj.7600294. PMC 514954. PMID 15241471.
• Michel D, Arsanto JP, Massey-Harroche D, Béclin C, Wijnholds J, Le Bivic A (2005). "PATJ connects and stabilizes apical and lateral components of tight junctions in human intestinal cells". J. Cell Sci. 118 (Pt 17): 4049–57. doi:10.1242/jcs.02528. PMID 16129888. S2CID 19826254.
• Stiffler MA, Grantcharova VP, Sevecka M, MacBeath G (2007). "Uncovering Quantitative Protein Interaction Networks for Mouse PDZ Domains using Protein Microarrays". J. Am. Chem. Soc. 128 (17): 5913–22. doi:10.1021/ja060943h. PMC 2533859. PMID 16637659.
• Wells CD, Fawcett JP, Traweger A, Yamanaka Y, Goudreault M, Elder K, Kulkarni S, Gish G, Virag C, Lim C, Colwill K, Starostine A, Metalnikov P, Pawson T (2006). "A Rich1/Amot complex regulates the Cdc42 GTPase and apical-polarity proteins in epithelial cells". Cell. 125 (3): 535–48. doi:10.1016/j.cell.2006.02.045. PMID 16678097. S2CID 18651810.

External links

• INADL human gene location in the UCSC Genome Browser.
• INADL human gene details in the UCSC Genome Browser.
• Overview of all the structural information available in the PDB for UniProt: Q8NI35 (InaD-like protein) at the PDBe-KB.