Janus Green B is a basic dye and vital stain used in histology. It is also used to stain mitochondria supravitally, as was introduced by Leonor Michaelis in 1900.[2]
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Names | |||
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IUPAC name
8-(4-Dimethylaminophenyl)diazenyl-N,N-diethyl-10-phenylphenazin-10-ium-2-amine chloride
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Other names
Diazin Green S
Union Green B | |||
Identifiers | |||
3D model (JSmol)
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ChEBI | |||
ChemSpider | |||
ECHA InfoCard | 100.018.814 | ||
EC Number |
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PubChem CID
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UNII | |||
CompTox Dashboard (EPA)
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Properties | |||
C30H31ClN6 | |||
Molar mass | 511.06 g/mol | ||
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
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The indicator Janus Green B changes colour according to the amount of oxygen present.[3] When oxygen is present, the indicator oxidizes to a blue colour. In the absence of oxygen, the indicator is reduced and changes to a pink colour.
References
edit- ^ Janus Green B, at Sigma-Aldrich
- ^ Michaelis, L. (1900). Die vitale Farbung, eine Darstellungsmethode der Zellgranula. Archiv für mikroskopische Anatomie 55:558-575(Also Tafel XXXII)
- ^ "Archived copy". Archived from the original on 2006-02-24. Retrieved 2007-10-31.
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: CS1 maint: archived copy as title (link)