John Roth may refer to:
- John Roth (clergyman) (1726–1791), Moravian clergyman
- John Roth (businessman) (born 1942), Canadian CEO of Nortel
- John K. Roth, American-based author, editor, and professor of philosophy of religion at Claremont McKenna College
- John D. Roth, editor of the Mennonite Quarterly Review
- John Roth (musician) (born 1967), guitarist with American band Winger
- John Roth (geneticist) (born 1939), American geneticist at University of California, Davis
- John Roth (politician), American politician from Michigan
- John P. Roth, American government official