Johnny Jump Up can refer to:
- A number of species of violet (Viola sp.) flower, including:
- Viola bicolor, also known as the American field pansy
- Viola cornuta, also known as the tufted pansy
- Viola pedunculata also known as the California golden violet
- Viola tricolor, also known as heartsease
- A local name for the blue-black grassquit bird
- Johnny Jump Up (song), an Irish folk song about strong cider