Last drop is often an allusion to the proverb "The last drop makes the cup run over". It may also refer to:
- The Last Drop (Leyster), a 1639 painting by Judith Leyster
- The Last Drop of Water, a 1911 American film by D.W. Griffth
- "Last Drop", a 2004 song by Kevin Lyttle
- The Last Drop, a 2006 British-Romanian WWII film by Colin Teague
- Every Last Drop, a 2008 horror novel by Charlie Huston in the Joe Pitt series