Murid gammaherpesvirus 68 (MuHV-68) is an isolate of the virus species Murid gammaherpesvirus 4, a member of the genus Rhadinovirus. It is a member of the subfamily Gammaherpesvirinae in the family of Herpesviridae. MuHV-68 serves as a model for study of human gammaherpesviruses which cause significant human disease including B-cell lymphoma and Kaposi's sarcoma. The WUMS strain of MuHV-68 was fully sequenced and annotated in 1997,[1] and the necessity of most of its genes in viral replication was characterized by random transposon mutagenesis.[2]
| Murid gammaherpesvirus 68 | |
|---|---|
Virus classification 
| |
| (unranked): | Virus |
| Realm: | Duplodnaviria |
| Kingdom: | Heunggongvirae |
| Phylum: | Peploviricota |
| Class: | Herviviricetes |
| Order: | Herpesvirales |
| Family: | Orthoherpesviridae |
| Genus: | Rhadinovirus |
| Species: | |
| Virus: | Murid gammaherpesvirus 68
|
Surface proteins
editAlpha-, beta-, and gammaherpesviruses display a heterodimer composed of glycoprotein H (gH) and glycoprotein L (gL) on their envelopes. This receptor is involved in the cell-to-cell transmission of the virus. Glycoprotein H has two conformations. Glycoprotein L is a chaperone protein which assures that gH takes on the correct conformation. Murid gammaherpesvirus 68 lack gL, gH misfolds. When alpha- or betaherpesviruses lack gL, they are noninfectious. Murid gammaherpesvirus 68 lacks gL, it remains infectious but is less able to bind to fibroblasts and epithelial cells.[3]
The open reading frame M7 of the MuHV-68 genome encodes for the surface receptor glycoprotein 150 (gp150). It is homologous to the Epstein-Barr virus membrane antigen gp350/220.[4] MuHV-68 is more closely related to the Kaposi's Sarcoma-associated herpesvirus (KSHV) than it is to the Epstein-Barr virus. Glycoprotein K8.1 is the KSHV homolog of MuHV-68 gp150.[5] MuHV-68 is a very close relative of MuHV-72. The MuHV-68 M7 gene differs from the corresponding MuHV-72 gene by five point mutations which alter four codons.[4] Glycoprotein 150 allows MuHV-68 to bind to B-cells.[5]
References
edit- ^ Herbet W. Virgin IV; Philip Latreille; Pamela Wamsley; Kymberlie Hallsworth; Karen E. Weck; Albert J. Dal Canto & Samuel H. Speck (August 1997). "Complete sequence and genomic analysis of murine gammaherpesvirus 68". Journal of Virology. 71 (8): 5894–904. doi:10.1128/JVI.71.8.5894-5904.1997. PMC 191845. PMID 9223479.
- ^ Moon Jung Song; Seungmin Hwang; Wendy H. Wong; Ting-Ting Wu; Sangmi Lee; Hsiang-I Liao & Ren Sun (March 2005). "Identification of viral genes essential for replication of murine γ-herpesvirus 68 using signature-tagged mutagenesis". Proceedings of the National Academy of Sciences of the United States of America. 102 (10): 3805–10. Bibcode:2005PNAS..102.3805S. doi:10.1073/pnas.0404521102. PMC 553290. PMID 15738413.
- ^ Laurent Gillet; Janet S. May; Susanna Colaco & Philip G. Stevenson (January 2007). "Glycoprotein L Disruption Reveals Two Functional Forms of the Murine Gammaherpesvirus 68 Glycoprotein H". Journal of Virology. 81 (1): 280–291. doi:10.1128/jvi.01616-06. PMC 1797276. PMID 17050601.
- ^ a b Macáková K, Matis J, Rezuchová I, Kúdela O, Raslová H, Kúdelová M (Jan 2003). "Murine gammaherpesvirus (MuHV) M7 gene encoding glycoprotein 150 (gp150): difference in the sequence between 72 and 68 strains". Virus Genes. 26 (1): 89–95. doi:10.1023/a:1022390407991. PMID 12683351. S2CID 34818785.
- ^ a b James P. Stewart; Ondine J. Silvia; Isobel M. D. Atkin; David J. Hughes; Bahram Ebrahimi & Heiko Adler (October 2004). "In Vivo Function of a Gammaherpesvirus Virion Glycoprotein: Influence on B-Cell Infection and Mononucleosis". Journal of Virology. 78 (19): 10449–10459. doi:10.1128/jvi.78.19.10449-10459.2004. PMC 516434. PMID 15367611.