The NMT1 RNA motif is a conserved RNA structure that was discovered by bioinformatics.[1] NMT1 motif RNAs are found in Pseudomonadota. There is also one NMT1 RNA in each of Bacteroidota and Actinomycetota, but these appear to be the result of recent horizontal gene transfer or sequence contamination before or during genome sequencing
| NMT1 | |
|---|---|
 Consensus secondary structure and sequence conservation of NMT1 RNA | |
| Identifiers | |
| Symbol | NMT1 |
| Rfam | RF03054 |
| Other data | |
| RNA type | Cis-reg |
| SO | SO:0005836 |
| PDB structures | PDBe |
NMT1 has been shown to act as part of a xanthane riboswitch that turns off gene expression when the ligand is bound.[2]
The NMT1 motif RNA were thought to likely function as cis-regulatory elements, in view of their positions upstream of protein-coding genes. Indeed, the RNAs are upstream of multiple genes that encode non-homologous proteins. If all examples of the RNA were upstream of homologous genes, there is the possibility that the RNAs were conserved in that position simply by inheritance. The non-homology of the genes downstream of NMT1 RNAs makes this scenario less likely.
The most common type of gene that is apparently regulated by NMT1 RNAs is NMT1. These genes are regulated by thiamin in yeast.[3] Another gene class frequently regulated by NMT1 RNAs are those encoding dioxygenase. However, the substrate specificity of these enzymes has not been predicted. Some NMT1 RNAs apparently regulate genes that encode deaminases. In a previous, unrelated study, some enzymes operated in vitro as deaminases of isoxanthopterin,[4] although it is not believed that this is their natural substrate. Proteins with similar sequences had mutations that resulted in uncertainty as to whether or not they would also use isoxanthopterin as a substrate.[4] These ambiguous proteins are regulated by NMT1 RNAs, e.g., with locus tags PputW619_2587 (in Pseudomonas putida W619), PP_3209 (in Pseudomonas putida KT2440) and Mmwyl1_4101 (in a Marinomonas species).
References
edit- ^ Weinberg Z, Lünse CE, Corbino KA, Ames TD, Nelson JW, Roth A, Perkins KR, Sherlock ME, Breaker RR (October 2017). "Detection of 224 candidate structured RNAs by comparative analysis of specific subsets of intergenic regions". Nucleic Acids Res. 45 (18): 10811–10823. doi:10.1093/nar/gkx699. PMC 5737381. PMID 28977401.
- ^ Yu, Diane; Breaker, Ronald R. (2020-04-28). "A bacterial riboswitch class senses xanthine and uric acid to regulate genes associated with purine oxidation". RNA. 26 (8): 960–968. doi:10.1261/rna.075218.120. ISSN 1469-9001. PMC 7373994. PMID 32345632.
- ^ Maundrell K (July 1990). "nmt1 of fission yeast. A highly transcribed gene completely repressed by thiamine". J. Biol. Chem. 265 (19): 10857–10864. doi:10.1016/S0021-9258(19)38525-4. PMID 2358444.
- ^ a b Hall RS, Agarwal R, Hitchcock D, Sauder JM, Burley SK, Swaminathan S, Raushel FM (May 2010). "Discovery and structure determination of the orphan enzyme isoxanthopterin deaminase". Biochemistry. 49 (20): 4374–4382. doi:10.1021/bi100252s. PMC 2892419. PMID 20415463.