Phosphoethanolamine/phosphocholine phosphatase (EC 3.1.3.75, PHOSPHO1, 3X11A; systematic name phosphoethanolamine phosphohydrolase) is an enzyme highly expressed in mineralizing cells .[1][2][3] This enzyme is implicated in bone and cartilage formation and catalyses the following chemical reactions:
| Phosphoethanolamine/phosphocholine phosphatase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC no. | 3.1.3.75 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| |||||||||
- (1) O-phosphoethanolamine + H2O ethanolamine + phosphate
- (2) phosphocholine + H2O choline + phosphate
The enzyme is a member of the haloacid dehalogenase superfamily. Like other members of this superfamily it requires a metal ion for catalysis, which is usually Mg2+, it is also active in the presence of Co2+ or Mn2+ but exhibits a lower specific activity with these metal ions.
References
edit- ^ Houston B, Seawright E, Jefferies D, Hoogland E, Lester D, Whitehead C, Farquharson C (January 1999). "Identification and cloning of a novel phosphatase expressed at high levels in differentiating growth plate chondrocytes". Biochimica et Biophysica Acta. 1448 (3): 500–6. doi:10.1016/s0167-4889(98)00153-0. PMID 9990301.
- ^ Stewart AJ, Schmid R, Blindauer CA, Paisey SJ, Farquharson C (December 2003). "Comparative modelling of human PHOSPHO1 reveals a new group of phosphatases within the haloacid dehalogenase superfamily". Protein Engineering. 16 (12): 889–95. doi:10.1093/protein/gzg126. PMID 14983068.
- ^ Roberts SJ, Stewart AJ, Sadler PJ, Farquharson C (August 2004). "Human PHOSPHO1 exhibits high specific phosphoethanolamine and phosphocholine phosphatase activities". The Biochemical Journal. 382 (Pt 1): 59–65. doi:10.1042/bj20040511. PMC 1133915. PMID 15175005.
External links
edit- Phosphoethanolamine/phosphocholine+phosphatase at the U.S. National Library of Medicine Medical Subject Headings (MeSH)
