Wikipedia

Pharmacogenomics

(Redirected from Slow metabolizer)
For the journal, see Pharmacogenomics (journal).

Pharmacogenomics, often abbreviated "PGx," is the study of the role of the genome in drug response. Its name (pharmaco- + genomics) reflects its combining of pharmacology and genomics. Pharmacogenomics analyzes how the genetic makeup of a patient affects their response to drugs.[1] It deals with the influence of acquired and inherited genetic variation on drug response, by correlating DNA mutations (including point mutations, copy number variations, and structural variations) with pharmacokinetic (drug absorption, distribution, metabolism, and elimination), pharmacodynamic (effects mediated through a drug's biological targets), and/or immunogenic endpoints.[2][3][4]

Pharmacogenomics aims to develop rational means to optimize drug therapy, with regard to the patients' genotype, to achieve maximum efficiency with minimal adverse effects.[5] It is hoped that by using pharmacogenomics, pharmaceutical drug treatments can deviate from what is dubbed as the "one-dose-fits-all" approach. Pharmacogenomics also attempts to eliminate trial-and-error in prescribing, allowing physicians to take into consideration their patient's genes, the functionality of these genes, and how this may affect the effectiveness of the patient's current or future treatments (and where applicable, provide an explanation for the failure of past treatments).[6][7] Such approaches promise the advent of precision medicine and even personalized medicine, in which drugs and drug combinations are optimized for narrow subsets of patients or even for each individual's unique genetic makeup.[8][9]

Whether used to explain a patient's response (or lack of it) to a treatment, or to act as a predictive tool, it hopes to achieve better treatment outcomes and greater efficacy, and reduce drug toxicities and adverse drug reactions (ADRs). For patients who do not respond to a treatment, alternative therapies can be prescribed that would best suit their requirements. In order to provide pharmacogenomic recommendations for a given drug, two possible types of input can be used: genotyping, or exome or whole genome sequencing.[10] Sequencing provides many more data points, including detection of mutations that prematurely terminate the synthesized protein (early stop codon).[10]

Pharmacogenetics vs. pharmacogenomics

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The term pharmacogenomics is often used interchangeably with pharmacogenetics. Although both terms relate to drug response based on genetic influences, there are differences between the two. Pharmacogenetics is limited to monogenic phenotypes (i.e., single gene-drug interactions). Pharmacogenomics refers to polygenic drug response phenotypes and encompasses transcriptomics, proteomics, and metabolomics.

Mechanisms of pharmacogenetic interactions

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Pharmacokinetics

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Pharmacokinetics involves the absorption, distribution, metabolism, and elimination of pharmaceutics. These processes are often facilitated by enzymes such as drug transporters or drug metabolizing enzymes (discussed in-depth below). Variation in DNA loci responsible for producing these enzymes can alter their expression or activity so that their functional status changes. An increase, decrease, or loss of function for transporters or metabolizing enzymes can ultimately alter the amount of medication in the body and at the site of action. This may result in deviation from the medication's therapeutic window and result in either toxicity or loss of effectiveness.

Drug-metabolizing enzymes

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The majority of clinically actionable pharmacogenetic variation occurs in genes that code for drug-metabolizing enzymes, including those involved in both phase I and phase II metabolism. The cytochrome P450 enzyme family is responsible for metabolism of 70-80% of all medications used clinically.[11] CYP3A4, CYP2C9, CYP2C19, and CYP2D6 are major CYP enzymes involved in drug metabolism and are all known to be highly polymorphic.[11] Additional drug-metabolizing enzymes that have been implicated in pharmacogenetic interactions include UGT1A1 (a UDP-glucuronosyltransferase), DPYD, and TPMT.[12]

Drug transporters

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Many medications rely on transporters to cross cellular membranes in order to move between body fluid compartments such as the blood, gut lumen, bile, urine, brain, and cerebrospinal fluid.[13] The major transporters include the solute carrier, ATP-binding cassette, and organic anion transporters.[13] Transporters that have been shown to influence response to medications include OATP1B1 (SLCO1B1) and breast cancer resistance protein (BCRP) (ABCG2).[14]

Pharmacodynamics

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Pharmacodynamics refers to the impact a medication has on the body, or its mechanism of action.

Drug targets

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Drug targets are the specific sites where a medication carries out its pharmacological activity. The interaction between the drug and this site results in a modification of the target that may include inhibition or potentiation.[15] Most of the pharmacogenetic interactions that involve drug targets are within the field of oncology and include targeted therapeutics designed to address somatic mutations (see also Cancer Pharmacogenomics). For example, EGFR inhibitors like gefitinib (Iressa) or erlotinib (Tarceva) are only indicated in patients carrying specific mutations to EGFR.[16][17]

Germline mutations in drug targets can also influence response to medications, though this is an emerging subfield within pharmacogenomics. One well-established gene-drug interaction involving a germline mutation to a drug target is warfarin (Coumadin) and VKORC1, which codes for vitamin K epoxide reductase (VKOR). Warfarin binds to and inhibits VKOR, which is an important enzyme in the vitamin K cycle.[18] Inhibition of VKOR prevents reduction of vitamin K, which is a cofactor required in the formation of coagulation factors II, VII, IX and X, and inhibitors protein C and S.[18][19]

Off-target sites

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Medications can have off-target effects (typically unfavorable) that arise from an interaction between the medication and/or its metabolites and a site other than the intended target.[20] Genetic variation in the off-target sites can influence this interaction. The main example of this type of pharmacogenomic interaction is glucose-6-phosphate-dehydrogenase (G6PD). G6PD is the enzyme involved in the first step of the pentose phosphate pathway which generates NADPH (from NADP). NADPH is required for the production of reduced glutathione in erythrocytes and it is essential for the function of catalase.[21] Glutathione and catalase protect cells from oxidative stress that would otherwise result in cell lysis. Certain variants in G6PD result in G6PD deficiency, in which cells are more susceptible to oxidative stress. When medications that have a significant oxidative effect are administered to individuals who are G6PD deficient, they are at an increased risk of erythrocyte lysis that presents as hemolytic anemia.[22]

Immunologic

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The human leukocyte antigen (HLA) system, also referred to as the major histocompatibility complex (MHC), is a complex of genes important for the adaptive immune system. Mutations in the HLA complex have been associated with an increased risk of developing hypersensitivity reactions in response to certain medications.[23]

Clinical pharmacogenomics resources

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Clinical Pharmacogenetics Implementation Consortium (CPIC)

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The Clinical Pharmacogenetics Implementation Consortium (CPIC) is "an international consortium of individual volunteers and a small dedicated staff who are interested in facilitating use of pharmacogenetic tests for patient care. CPIC’s goal is to address barriers to clinical implementation of pharmacogenetic tests by creating, curating, and posting freely available, peer-reviewed, evidence-based, updatable, and detailed gene/drug clinical practice guidelines. CPIC guidelines follow standardized formats, include systematic grading of evidence and clinical recommendations, use standardized terminology, are peer-reviewed, and are published in a journal (in partnership with Clinical Pharmacology and Therapeutics) with simultaneous posting to cpicpgx.org, where they are regularly updated."[12]

The CPIC guidelines are "designed to help clinicians understand HOW available genetic test results should be used to optimize drug therapy, rather than WHETHER tests should be ordered. A key assumption underlying the CPIC guidelines is that clinical high-throughput and pre-emptive (pre-prescription) genotyping will become more widespread, and that clinicians will be faced with having patients’ genotypes available even if they have not explicitly ordered a test with a specific drug in mind. CPIC's guidelines, processes and projects have been endorsed by several professional societies."[12]

U.S. Food and Drug Administration

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Table of Pharmacogenetic Associations

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In February 2020 the FDA published the Table of Pharmacogenetic Associations.[24] For the gene-drug pairs included in the table, "the FDA has evaluated and believes there is sufficient scientific evidence to suggest that subgroups of patients with certain genetic variants, or genetic variant-inferred phenotypes (such as affected subgroup in the table below), are likely to have altered drug metabolism, and in certain cases, differential therapeutic effects, including differences in risks of adverse events."[25]

"The information in this Table is intended primarily for prescribers, and patients should not adjust their medications without consulting their prescriber. This version of the table is limited to pharmacogenetic associations that are related to drug metabolizing enzyme gene variants, drug transporter gene variants, and gene variants that have been related to a predisposition for certain adverse events. The FDA recognizes that various other pharmacogenetic associations exist that are not listed here, and this table will be updated periodically with additional pharmacogenetic associations supported by sufficient scientific evidence."[25]

Table of Pharmacogenomic Biomarkers in Drug Labeling

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The FDA Table of Pharmacogenomic Biomarkers in Drug Labeling lists FDA-approved drugs with pharmacogenomic information found in the drug labeling. "Biomarkers in the table include but are not limited to germline or somatic gene variants (polymorphisms, mutations), functional deficiencies with a genetic etiology, gene expression differences, and chromosomal abnormalities; selected protein biomarkers that are used to select treatments for patients are also included."[26]

PharmGKB

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The Pharmacogenomics Knowledgebase (PharmGKB) is an "NIH-funded resource that provides information about how human genetic variation affects response to medications. PharmGKB collects, curates and disseminates knowledge about clinically actionable gene-drug associations and genotype-phenotype relationships."[27]

Commercial Pharmacogenetic Testing Laboratories

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There are many commercial laboratories around the world who offer pharmacogenomic testing as a laboratory developed test (LDTs). The tests offered can vary significantly from one lab to another, including genes and alleles tested for, phenotype assignment, and any clinical annotations provided. With the exception of a few direct-to-consumer tests, all pharmacogenetic testing requires an order from an authorized healthcare professional. In order for the results to be used in a clinical setting in the United States, the laboratory performing the test much be CLIA-certified. Other regulations may vary by country and state.

 
Final consensus terms for allele functional status and phenotype

Direct-to-Consumer Pharmacogenetic Testing

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Direct-to-consumer (DTC) pharmacogenetic tests allow consumers to obtain pharmacogenetic testing without an order from a prescriber. DTC pharmacogenetic tests are generally reviewed by the FDA to determine the validity of test claims.[28] The FDA maintains a list of DTC genetic tests that have been approved.

Common Pharmacogenomic-Specific Nomenclature

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Genotype

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There are multiple ways to represent a pharmacogenomic genotype. A commonly used nomenclature system is to report haplotypes using a star (*) allele (e.g., CYP2C19 *1/*2). Single-nucleotide polymorphisms (SNPs) may be described using their assignment reference SNP cluster ID (rsID) or based on the location of the base pair or amino acid impacted.[29]

Phenotype

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In 2017 CPIC published results of an expert survey to standardize terms related to clinical pharmacogenetic test results.[30] Consensus for terms to describe allele functional status, phenotype for drug metabolizing enzymes, phenotype for drug transporters, and phenotype for high-risk genotype status was reached.

Applications

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The list below provides a few more commonly known applications of pharmacogenomics:[31]

  • Improve drug safety, and reduce ADRs;
  • Tailor treatments to meet patients' unique genetic pre-disposition, identifying optimal dosing;
  • Improve drug discovery targeted to human disease; and
  • Improve proof of principle for efficacy trials.

Pharmacogenomics may be applied to several areas of medicine, including pain management, cardiology, oncology, and psychiatry. A place may also exist in forensic pathology, in which pharmacogenomics can be used to determine the cause of death in drug-related deaths where no findings emerge using autopsy.[citation needed]

In cancer treatment, pharmacogenomics tests are used to identify which patients are most likely to respond to certain cancer drugs. In behavioral health, pharmacogenomic tests provide tools for physicians and care givers to better manage medication selection and side effect amelioration. Pharmacogenomics is also known as companion diagnostics, meaning tests being bundled with drugs. Examples include KRAS test with cetuximab and EGFR test with gefitinib. Beside efficacy, germline pharmacogenetics can help to identify patients likely to undergo severe toxicities when given cytotoxics showing impaired detoxification in relation with genetic polymorphism, such as canonical 5-FU.[32] In particular, genetic deregulations affecting genes coding for DPD, UGT1A1, TPMT, CDA and CYP2D6 are now considered as critical issues for patients treated with 5-FU/capecitabine, irinotecan, mercaptopurine/azathioprine, gemcitabine/capecitabine/AraC and tamoxifen, respectively.[33]

In cardiovascular disorders, the main concern is response to drugs including warfarin, clopidogrel, beta blockers, and statins.[10] In patients with CYP2C19, who take clopidogrel, cardiovascular risk is elevated, leading to medication package insert updates by regulators.[34] In patients with type 2 diabetes, haptoglobin (Hp) genotyping shows an effect on cardiovascular disease, with Hp2-2 at higher risk and supplemental vitamin E reducing risk by affecting HDL.[35]

In psychiatry, as of 2010, research has focused particularly on 5-HTTLPR and DRD2.[36]

Clinical implementation

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Further information: Education in personalized medicine

Initiatives to spur adoption by clinicians include the Ubiquitous Pharmacogenomics (U-PGx) program in Europe and the Clinical Pharmacogenetics Implementation Consortium (CPIC) in the United States.[37] In a 2017 survey of European clinicians, in the prior year two-thirds had not ordered a pharmacogenetic test.[38]

In 2010, Vanderbilt University Medical Center launched Pharmacogenomic Resource for Enhanced Decisions in Care and Treatment (PREDICT);[39] in 2015 survey, two-thirds of the clinicians had ordered a pharmacogenetic test.[40]

In 2019, the largest private health insurer, UnitedHealthcare, announced that it would pay for genetic testing to predict response to psychiatric drugs.[41]

In 2020, Canada's 4th largest health and dental insurer, Green Shield Canada, announced that it would pay for pharmacogenetic testing and its associated clinical decision support software to optimize and personalize mental health prescriptions.[42]

Reduction of polypharmacy

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A potential role for pharmacogenomics is to reduce the occurrence of polypharmacy: it is theorized that with tailored drug treatments, patients will not need to take several medications to treat the same condition. Thus they could potentially reduce the occurrence of adverse drug reactions, improve treatment outcomes, and save costs by avoiding purchase of some medications. For example, maybe due to inappropriate prescribing, psychiatric patients tend to receive more medications than age-matched non-psychiatric patients.[43]

The need for pharmacogenomically tailored drug therapies may be most evident in a survey conducted by the Slone Epidemiology Center at Boston University from February 1998 to April 2007. The study elucidated that an average of 82% of adults in the United States are taking at least one medication (prescription or nonprescription drug, vitamin/mineral, herbal/natural supplement), and 29% are taking five or more. The study suggested that those aged 65 years or older continue to be the biggest consumers of medications, with 17-19% in this age group taking at least ten medications in a given week. Polypharmacy has also shown to have increased since 2000 from 23% to 29%.[44]

Example case studies

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Case A – Antipsychotic adverse reaction[45]

Patient A has schizophrenia. Their treatment included a combination of ziprasidone, olanzapine, trazodone and benztropine. The patient experienced dizziness and sedation, so they were tapered off ziprasidone and olanzapine, and transitioned to quetiapine. Trazodone was discontinued. The patient then experienced excessive sweating, tachycardia and neck pain, gained considerable weight and had hallucinations. Five months later, quetiapine was tapered and discontinued, with ziprasidone re-introduced into their treatment, due to the excessive weight gain. Although the patient lost the excessive weight they had gained, they then developed muscle stiffness, cogwheeling, tremors and night sweats. When benztropine was added they experienced blurry vision. After an additional five months, the patient was switched from ziprasidone to aripiprazole. Over the course of 8 months, patient A gradually experienced more weight gain and sedation, and developed difficulty with their gait, stiffness, cogwheeling and dyskinetic ocular movements. A pharmacogenomics test later proved the patient had a CYP2D6 *1/*41, which has a predicted phenotype of IM and CYP2C19 *1/*2 with a predicted phenotype of IM as well.

Case B – Pain Management[46]

Patient B is a woman who gave birth by caesarian section. Her physician prescribed codeine for post-caesarian pain. She took the standard prescribed dose, but she experienced nausea and dizziness while she was taking codeine. She also noticed that her breastfed infant was lethargic and feeding poorly. When the patient mentioned these symptoms to her physician, they recommended that she discontinue codeine use. Within a few days, both the patient's and her infant's symptoms were no longer present. It is assumed that if the patient had undergone a pharmacogenomic test, it would have revealed she may have had a duplication of the gene CYP2D6, placing her in the Ultra-rapid metabolizer (UM) category, explaining her reactions to codeine use.

Case C – FDA Warning on Codeine Overdose for Infants[47]

On February 20, 2013, the FDA released a statement addressing a serious concern regarding the connection between children who are known as CYP2D6 UM, and fatal reactions to codeine following tonsillectomy and/or adenoidectomy (surgery to remove the tonsils and/or adenoids). They released their strongest Boxed Warning to elucidate the dangers of CYP2D6 UMs consuming codeine. Codeine is converted to morphine by CYP2D6, and those who have UM phenotypes are in danger of producing large amounts of morphine due to the increased function of the gene. The morphine can elevate to life-threatening or fatal amounts, as became evident with the death of three children in August 2012.

Challenges

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Consecutive phases and associated challenges in Pharmacogenomics.[48]

Although there appears to be a general acceptance of the basic tenet of pharmacogenomics amongst physicians and healthcare professionals,[49] several challenges exist that slow the uptake, implementation, and standardization of pharmacogenomics. Some of the concerns raised by physicians include:[50][49][51]

  • Limitation on how to apply the test into clinical practices and treatment;
  • A general feeling of lack of availability of the test;
  • The understanding and interpretation of evidence-based research;
  • Combining test results with other patient data for prescription optimization; and
  • Ethical, legal and social issues.

Issues surrounding the availability of the test include:[48]

  • The lack of availability of scientific data: Although there are a considerable number of drug-metabolizing enzymes involved in the metabolic pathways of drugs, only a fraction have sufficient scientific data to validate their use within a clinical setting; and
  • Demonstrating the cost-effectiveness of pharmacogenomics: Publications for the pharmacoeconomics of pharmacogenomics are scarce, therefore sufficient evidence does not at this time exist to validate the cost-effectiveness and cost-consequences of the test.

Although other factors contribute to the slow progression of pharmacogenomics (such as developing guidelines for clinical use), the above factors appear to be the most prevalent. Increasingly substantial evidence and industry body guidelines for clinical use of pharmacogenetics have made it a population wide approach to precision medicine. Cost, reimbursement, education, and easy use at the point of care remain significant barriers to widescale adoption.

Controversies

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Race-based medicine

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There has been call to move away from race and ethnicity in medicine and instead use genetic ancestry as a way to categorize patients.[52] Some alleles that vary in frequency between specific populations have been shown to be associated with differential responses to specific drugs. As a result, some disease-specific guidelines only recommend pharmacogenetic testing for populations where high-risk alleles are more common[53] and, similarly, certain insurance companies will only pay for pharmacogenetic testing for beneficiaries of high-risk populations.[54]

Genetic exceptionalism

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In the early 2000s, handling genetic information as exceptional, including legal or regulatory protections, garnered strong support. It was argued that genomic information may need special policy and practice protections within the context of electronic health records (EHRs).[55] In 2008, the Genetic Information Nondiscrimination Act (GINA) was enacted to protect patients from health insurance companies discriminating against an individual based on genetic information.[56][57]

More recently it has been argued that genetic exceptionalism is past its expiration date as we move into a blended genomic/big data era of medicine, yet exceptionalism practices continue to permeate clinical healthcare today.[58][59] Garrison et al. recently relayed a call to action to update verbiage from genetic exceptionalism to genomic contextualism in that we recognize a fundamental duality of genetic information.[60] This allows room in the argument for different types of genetic information to be handled differently while acknowledging that genomic information is similar and yet distinct from other health-related information.[60] Genomic contextualism would allow for a case-by-case analysis of the technology and the context of its use (e.g., clinical practice, research, secondary findings).

Others argue that genetic information is indeed distinct from other health-related information but not to the extent of requiring legal/regulatory protections, similar to other sensitive health-related data such as HIV status.[61] Additionally, Evans et al. argue that the EHR has sufficient privacy standards to hold other sensitive information such as social security numbers and that the fundamental nature of an EHR is to house highly personal information.[58] Similarly, a systematic review reported that the public had concern over privacy of genetic information, with 60% agreeing that maintaining privacy was not possible; however, 96% agreed that a direct-to-consumer testing company had protected their privacy, with 74% saying their information would be similarly or better protected in an EHR. With increasing technological capabilities in EHRs, it is possible to mask or hide genetic data from subsets of providers and there is not consensus on how, when, or from whom genetic information should be masked.[55][62] Rigorous protection and masking of genetic information is argued to impede further scientific progress and clinical translation into routine clinical practices.[63]

History

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Pharmacogenomics was first recognized by Pythagoras around 510 BC when he made a connection between the dangers of fava bean ingestion with hemolytic anemia and oxidative stress. In the 1950s, this identification was validated and attributed to deficiency of G6PD and is called favism.[64][65] Although the first official publication was not until 1961,[66] the unofficial beginnings of this science were around the 1950s. Reports of prolonged paralysis and fatal reactions linked to genetic variants in patients who lacked butyrylcholinesterase ('pseudocholinesterase') following succinylcholine injection during anesthesia were first reported in 1956.[2][67] The term pharmacogenetics was first coined in 1959 by Friedrich Vogel of Heidelberg, Germany (although some papers suggest it was 1957 or 1958).[68] In the late 1960s, twin studies supported the inference of genetic involvement in drug metabolism, with identical twins sharing remarkable similarities in drug response compared to fraternal twins.[69] The term pharmacogenomics first began appearing around the 1990s.[64]

The first FDA approval of a pharmacogenetic test was in 2005[9] (for alleles in CYP2D6 and CYP2C19)

Future

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Computational advances have enabled cheaper and faster sequencing.[70] Research has focused on combinatorial chemistry,[71] genomic mining, omic technologies, and high throughput screening.

As the cost per genetic test decreases, the development of personalized drug therapies will increase.[72] Technology now allows for genetic analysis of hundreds of target genes involved in medication metabolism and response in less than 24 hours for under $1,000. This a huge step towards bringing pharmacogenetic technology into everyday medical decisions. Likewise, companies like deCODE genetics, MD Labs Pharmacogenetics, Navigenics and 23andMe offer genome scans. The companies use the same genotyping chips that are used in GWAS studies and provide customers with a write-up of individual risk for various traits and diseases and testing for 500,000 known SNPs. Costs range from $995 to $2500 and include updates with new data from studies as they become available. The more expensive packages even included a telephone session with a genetics counselor to discuss the results.[73]

Ethics

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Pharmacogenetics has become a controversial issue in the area of bioethics. Privacy and confidentiality are major concerns.[74] The evidence of benefit or risk from a genetic test may only be suggestive, which could cause dilemmas for providers.[74]: 145  Drug development may be affected, with rare genetic variants possibly receiving less research.[74] Access and patient autonomy are also open to discussion.[75]: 680 

Web-based resources

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Web Resources for Pharmacogenomics[76][77]
Data source Main use Citation
PharmVar A central repository for pharmacogene variation that focuses on haplotype structure and allelic variation [78]
SuperCYP Bioinformatics Tool Containing 1170 drugs with more than 3800 interactions, and approximately 2000 known SNPs. These SNPs are listed and ordered according to their effect on expression and/or activity. [79]
PharmGKB The Pharmacogenomics Knowledge Base (PharmGKB) is an interactive tool for researchers investigating how genetic variation affects drug response. [80]
dbSNP database A repository of SNPs and other variants that have been reported after discovery, compiled and officially named. These are SNPs across the board. [81][82]
FINDbase Repository of allele frequencies of pharmacogenetic markers in different populations [83]
Pharmacogenomics Biomarkers in Drug Labelling A table that identifies which FDA-approved drugs have pharmacogenomics-related warning labels [84]
SNPedia A wiki-based bioinformatics database of SNPs [85][86]
Pharmacogenomics Research Network (PGRN) The PGRN hosts resources and information to stimulate collaborative research in pharmacogenomics and precision medicine. [87]

See also

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References

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  1. ^ Ermak G (2015). Emerging Medical Technologies. World Scientific. ISBN 978-981-4675-80-2.
  2. ^ a b Johnson JA (November 2003). "Pharmacogenetics: potential for individualized drug therapy through genetics". Trends in Genetics. 19 (11): 660–666. doi:10.1016/j.tig.2003.09.008. PMID 14585618. S2CID 15195039.
  3. ^ "Center for Pharmacogenomics and Individualized Therapy". Unc Eshelman School of Pharmacy. Retrieved 2014-06-25.
  4. ^ "overview of pharmacogenomics". Up-to-Date. May 16, 2014. Retrieved 2014-06-25.
  5. ^ Becquemont L (June 2009). "Pharmacogenomics of adverse drug reactions: practical applications and perspectives". Pharmacogenomics. 10 (6): 961–969. doi:10.2217/pgs.09.37. PMID 19530963.
  6. ^ Sheffield LJ, Phillimore HE (May 2009). "Clinical use of pharmacogenomic tests in 2009". The Clinical Biochemist. Reviews. 30 (2): 55–65. PMC 2702214. PMID 19565025.
  7. ^ Hauser AS, Chavali S, Masuho I, Jahn LJ, Martemyanov KA, Gloriam DE, Babu MM (January 2018). "Pharmacogenomics of GPCR Drug Targets". Cell. 172 (1–2): 41–54.e19. doi:10.1016/j.cell.2017.11.033. PMC 5766829. PMID 29249361.
  8. ^ "Guidance for Industry Pharmacogenomic Data Submissions" (PDF). U.S. Food and Drug Administration. March 2005. Retrieved 2008-08-27.
  9. ^ a b Squassina A, Manchia M, Manolopoulos VG, Artac M, Lappa-Manakou C, Karkabouna S, et al. (August 2010). "Realities and expectations of pharmacogenomics and personalized medicine: impact of translating genetic knowledge into clinical practice". Pharmacogenomics. 11 (8): 1149–1167. doi:10.2217/pgs.10.97. PMID 20712531.
  10. ^ a b c Huser V, Cimino JJ (2013). "Providing pharmacogenomics clinical decision support using whole genome sequencing data as input". AMIA Joint Summits on Translational Science Proceedings. AMIA Joint Summits on Translational Science. 2013: 81. PMID 24303303.
  11. ^ a b Zanger, Ulrich M.; Schwab, Matthias (2013-04-01). "Cytochrome P450 enzymes in drug metabolism: Regulation of gene expression, enzyme activities, and impact of genetic variation". Pharmacology & Therapeutics. 138 (1): 103–141. doi:10.1016/j.pharmthera.2012.12.007. ISSN 0163-7258. PMID 23333322.
  12. ^ a b c "Clinical Pharmacogenetics Implementation Consortium". cpicpgx.org. Retrieved 2022-12-13.
  13. ^ a b Nigam SK (January 2015). "What do drug transporters really do?". Nature Reviews. Drug Discovery. 14 (1): 29–44. doi:10.1038/nrd4461. PMC 4750486. PMID 25475361.
  14. ^ Cooper-DeHoff, Rhonda M.; Niemi, Mikko; Ramsey, Laura B.; Luzum, Jasmine A.; Tarkiainen, E. Katriina; Straka, Robert J.; Gong, Li; Tuteja, Sony; Wilke, Russell A.; Wadelius, Mia; Larson, Eric A.; Roden, Dan M.; Klein, Teri E.; Yee, Sook Wah; Krauss, Ronald M. (May 2022). "The Clinical Pharmacogenetics Implementation Consortium Guideline for SLCO1B1, ABCG2, and CYP2C9 genotypes and Statin-Associated Musculoskeletal Symptoms". Clinical Pharmacology and Therapeutics. 111 (5): 1007–1021. doi:10.1002/cpt.2557. ISSN 1532-6535. PMC 9035072. PMID 35152405.
  15. ^ Zanders, Edward D. (2011-03-21). "Introduction to Drugs and Drug Targets". The Science and Business of Drug Discovery. pp. 11–27. doi:10.1007/978-1-4419-9902-3_2. ISBN 978-1-4419-9901-6. PMC 7120710.
  16. ^ Iressa [package insert]. Wilmington, DE: Astra Zeneca; 2021.
  17. ^ Tarceva [package insert]. Northbrook, IL: OSI Pharmaceuticals, LLC; 2016. https://www.accessdata.fda.gov/drugsatfda_docs/label/2016/021743s025lbl.pdf
  18. ^ a b Oldenburg, Johannes; Marinova, Milka; Müller-Reible, Clemens; Watzka, Matthias (2008). "The vitamin K cycle". Vitamins and Hormones. 78: 35–62. doi:10.1016/S0083-6729(07)00003-9. ISBN 9780123741134. ISSN 0083-6729. PMID 18374189.
  19. ^ Mijares, M. E.; Nagy, E.; Guerrero, B.; Arocha-Piñango, C. L. (September 1998). "[Vitamin K: biochemistry, function, and deficiency. Review]". Investigacion Clinica. 39 (3): 213–229. ISSN 0535-5133. PMID 9780555.
  20. ^ Rudmann, Daniel G. (February 2013). "On-target and off-target-based toxicologic effects". Toxicologic Pathology. 41 (2): 310–314. doi:10.1177/0192623312464311. ISSN 1533-1601. PMID 23085982. S2CID 11858945.
  21. ^ Recht, Judith; Chansamouth, Vilada; White, Nicholas J.; Ashley, Elizabeth A. (2022-05-03). "Nitrofurantoin and glucose-6-phosphate dehydrogenase deficiency: a safety review". JAC-Antimicrobial Resistance. 4 (3): dlac045. doi:10.1093/jacamr/dlac045. ISSN 2632-1823. PMC 9070801. PMID 35529053.
  22. ^ Gammal, Roseann S.; Pirmohamed, Munir; Somogyi, Andrew A.; Morris, Sarah A.; Formea, Christine M.; Elchynski, Amanda L.; Oshikoya, Kazeem A.; McLeod, Howard L.; Haidar, Cyrine E.; Whirl-Carrillo, Michelle; Klein, Teri E.; Caudle, Kelly E.; Relling, Mary V. (May 2023). "Expanded Clinical Pharmacogenetics Implementation Consortium Guideline for Medication Use in the Context of G6PD Genotype". Clinical Pharmacology & Therapeutics. 113 (5): 973–985. doi:10.1002/cpt.2735. ISSN 0009-9236. PMC 10281211. PMID 36049896.
  23. ^ Pavlos R, Mallal S, Phillips E (August 2012). "HLA and pharmacogenetics of drug hypersensitivity". Pharmacogenomics. 13 (11): 1285–1306. doi:10.2217/pgs.12.108. PMID 22920398.
  24. ^ Office of the Commissioner (2020-03-24). "FDA Announces Collaborative Review of Scientific Evidence to Support Associations Between Genetic Information and Specific Medications". FDA. Retrieved 2022-12-13.
  25. ^ a b Center for Devices and Radiological Health (2022-10-26). "Table of Pharmacogenetic Associations". FDA.
  26. ^ Center for Drug Evaluation and Research (2022-08-11). "Table of Pharmacogenomic Biomarkers in Drug Labeling". FDA.
  27. ^ "PharmGKB". PharmGKB. Retrieved 2022-12-13.
  28. ^ Center for Drug Evaluation and Research (2021-06-25). "Direct-to-Consumer Tests". FDA.
  29. ^ Poo DC, Cai S, Mah JT (November 2011). "UASIS: Universal Automatic SNP Identification System". BMC Genomics. 12 (Suppl 3): S9. doi:10.1186/1471-2164-12-S3-S9. PMC 3333510. PMID 22369494.
  30. ^ Caudle KE, Dunnenberger HM, Freimuth RR, Peterson JF, Burlison JD, Whirl-Carrillo M, et al. (February 2017). "Standardizing terms for clinical pharmacogenetic test results: consensus terms from the Clinical Pharmacogenetics Implementation Consortium (CPIC)". Genetics in Medicine. 19 (2): 215–223. doi:10.1038/gim.2016.87. PMC 5253119. PMID 27441996.
  31. ^ Cohen N (November 2008). Pharmacogenomics and Personalized Medicine (Methods in Pharmacology and Toxicology). Totowa, NJ: Humana Press. p. 6. ISBN 978-1934115046.
  32. ^ Ciccolini J, Gross E, Dahan L, Lacarelle B, Mercier C (October 2010). "Routine dihydropyrimidine dehydrogenase testing for anticipating 5-fluorouracil-related severe toxicities: hype or hope?". Clinical Colorectal Cancer. 9 (4): 224–228. doi:10.3816/CCC.2010.n.033. PMID 20920994.
  33. ^ Yang CG, Ciccolini J, Blesius A, Dahan L, Bagarry-Liegey D, Brunet C, et al. (January 2011). "DPD-based adaptive dosing of 5-FU in patients with head and neck cancer: impact on treatment efficacy and toxicity". Cancer Chemotherapy and Pharmacology. 67 (1): 49–56. doi:10.1007/s00280-010-1282-4. PMID 20204365. S2CID 25362813.
  34. ^ Dean L (2012). "Clopidogrel Therapy and CYP2C19 Genotype". In Pratt VM, McLeod HL, Rubinstein WS, et al. (eds.). Medical Genetics Summaries. National Center for Biotechnology Information (NCBI). PMID 28520346. Bookshelf ID: NBK84114.
  35. ^ Bale BF, Doneen AL, Vigerust DJ (2018). "Precision Healthcare of Type 2 Diabetic Patients Through Implementation of Haptoglobin Genotyping". Frontiers in Cardiovascular Medicine. 5: 141. doi:10.3389/fcvm.2018.00141. PMC 6198642. PMID 30386783.
  36. ^ Malhotra AK (2010). "The state of pharmacogenetics". Psychiatr Times. 27 (4): 38–41, 62.
  37. ^ Williams MS (August 2019). "Early Lessons from the Implementation of Genomic Medicine Programs". Annual Review of Genomics and Human Genetics. 20 (1): 389–411. doi:10.1146/annurev-genom-083118-014924. PMID 30811224. S2CID 73460688.
  38. ^ Just KS, Steffens M, Swen JJ, Patrinos GP, Guchelaar HJ, Stingl JC (October 2017). "Medical education in pharmacogenomics-results from a survey on pharmacogenetic knowledge in healthcare professionals within the European pharmacogenomics clinical implementation project Ubiquitous Pharmacogenomics (U-PGx)". European Journal of Clinical Pharmacology. 73 (10): 1247–1252. doi:10.1007/s00228-017-2292-5. PMC 5599468. PMID 28669097.
  39. ^ Carlson B (2012). "Vanderbilt pioneers bedside genetics". Biotechnology Healthcare. 9 (2): 31–32. PMC 3411230. PMID 22876213.
  40. ^ Peterson JF, Field JR, Shi Y, Schildcrout JS, Denny JC, McGregor TL, et al. (August 2016). "Attitudes of clinicians following large-scale pharmacogenomics implementation". The Pharmacogenomics Journal. 16 (4): 393–398. doi:10.1038/tpj.2015.57. PMC 4751074. PMID 26261062.
  41. ^ "Pharmacogenetic test makers cheer UnitedHealth coverage. Other payers aren't there yet". MedTech Dive. Retrieved 2019-12-29.
  42. ^ "Personalized drug treatment through pharmacogenetics".
  43. ^ Ritsner M (2013). Polypharmacy in Psychiatry Practice, Volume I. Multiple Medication Strategies. Dordrecht: Springer Science and Business Media. ISBN 978-94-007-5804-9.
  44. ^ "Patterns of Medication Use in the United States". Boston University, Slone Epidemiology Center. 2006.
  45. ^ Foster A, Wang Z, Usman M, Stirewalt E, Buckley P (December 2007). "Pharmacogenetics of antipsychotic adverse effects: Case studies and a literature review for clinicians". Neuropsychiatric Disease and Treatment. 3 (6): 965–973. doi:10.2147/ndt.s1752. PMC 2656342. PMID 19300635.
  46. ^ "Pharmacogenetics: increasing the safety and effectiveness of drug therapy [Brochure]" (PDF). American Medical Association. 2011.
  47. ^ "FDA Drug Safety Communication: Safety review update of codeine use in children; new Boxed Warning and Contraindication on use after tonsillectomy and/or adenoidectomy". United States Food and Drug Administration. 2013-02-20.
  48. ^ a b Swen JJ, Huizinga TW, Gelderblom H, de Vries EG, Assendelft WJ, Kirchheiner J, Guchelaar HJ (August 2007). "Translating pharmacogenomics: challenges on the road to the clinic". PLOS Medicine. 4 (8): e209. doi:10.1371/journal.pmed.0040209. PMC 1945038. PMID 17696640.
  49. ^ a b Stanek EJ, Sanders CL, Taber KA, Khalid M, Patel A, Verbrugge RR, et al. (March 2012). "Adoption of pharmacogenomic testing by US physicians: results of a nationwide survey". Clinical Pharmacology and Therapeutics. 91 (3): 450–458. doi:10.1038/clpt.2011.306. PMID 22278335. S2CID 21366195.
  50. ^ "Center for Genetics Education".[permanent dead link‍]
  51. ^ Ma JD, Lee KC, Kuo GM (August 2012). "Clinical application of pharmacogenomics". Journal of Pharmacy Practice. 25 (4): 417–427. doi:10.1177/0897190012448309. PMID 22689709. S2CID 1212666.
  52. ^ Borrell LN, Elhawary JR, Fuentes-Afflick E, Witonsky J, Bhakta N, Wu AH, et al. (February 2021). "Race and Genetic Ancestry in Medicine - A Time for Reckoning with Racism". The New England Journal of Medicine. 384 (5): 474–480. doi:10.1056/NEJMms2029562. PMC 8979367. PMID 33406325.
  53. ^ FitzGerald JD, Dalbeth N, Mikuls T, Brignardello-Petersen R, Guyatt G, Abeles AM, et al. (June 2020). "2020 American College of Rheumatology Guideline for the Management of Gout". Arthritis Care & Research. 72 (6): 744–760. doi:10.1002/acr.24180. hdl:2027.42/155497. PMC 10563586. PMID 32391934. S2CID 218583019.
  54. ^ "LCD - Molecular Pathology Procedures (L35000)". www.cms.gov. Retrieved 2022-12-13.
  55. ^ a b McGuire AL, Fisher R, Cusenza P, Hudson K, Rothstein MA, McGraw D, et al. (July 2008). "Confidentiality, privacy, and security of genetic and genomic test information in electronic health records: points to consider". Genetics in Medicine. 10 (7): 495–499. doi:10.1097/gim.0b013e31817a8aaa. PMID 18580687. S2CID 29833634.
  56. ^ "The Genetic Information Nondiscrimination Act of 2008". US EEOC. Retrieved 2022-12-13.
  57. ^ Dressler LG, Terry SF (November 2009). "How will GINA influence participation in pharmacogenomics research and clinical testing?". Clinical Pharmacology and Therapeutics. 86 (5): 472–475. doi:10.1038/clpt.2009.146. PMID 19844223. S2CID 205121202.
  58. ^ a b Evans JP, Burke W (July 2008). "Genetic exceptionalism. Too much of a good thing?". Genetics in Medicine. 10 (7): 500–501. doi:10.1097/GIM.0b013e31817f280a. PMID 18580684. S2CID 32998031.
  59. ^ Murray TH (January 2019). "Is Genetic Exceptionalism Past Its Sell-By Date? On Genomic Diaries, Context, and Content". The American Journal of Bioethics. 19 (1): 13–15. doi:10.1080/15265161.2018.1552038. PMID 30676900. S2CID 59250873.
  60. ^ a b Garrison NA, Brothers KB, Goldenberg AJ, Lynch JA (January 2019). "Genomic Contextualism: Shifting the Rhetoric of Genetic Exceptionalism". The American Journal of Bioethics. 19 (1): 51–63. doi:10.1080/15265161.2018.1544304. PMC 6397766. PMID 30676903.
  61. ^ Sulmasy DP (May 2015). "Naked bodies, naked genomes: the special (but not exceptional) nature of genomic information". Genetics in Medicine. 17 (5): 331–336. doi:10.1038/gim.2014.111. PMID 25232853. S2CID 34092673.
  62. ^ Caraballo PJ, Sutton JA, Giri J, Wright JA, Nicholson WT, Kullo IJ, et al. (January 2020). "Integrating pharmacogenomics into the electronic health record by implementing genomic indicators". Journal of the American Medical Informatics Association. 27 (1): 154–158. doi:10.1093/jamia/ocz177. PMC 6913212. PMID 31591640.
  63. ^ Martani A, Geneviève LD, Pauli-Magnus C, McLennan S, Elger BS (2019-12-20). "Regulating the Secondary Use of Data for Research: Arguments Against Genetic Exceptionalism". Frontiers in Genetics. 10: 1254. doi:10.3389/fgene.2019.01254. PMC 6951399. PMID 31956328.
  64. ^ a b Pirmohamed M (October 2001). "Pharmacogenetics and pharmacogenomics". British Journal of Clinical Pharmacology. 52 (4): 345–347. doi:10.1046/j.0306-5251.2001.01498.x. PMC 2014592. PMID 11678777.
  65. ^ Prasad K (January 2009). "Role of regulatory agencies in translating pharmacogenetics to the clinics". Clinical Cases in Mineral and Bone Metabolism. 6 (1): 29–34. PMC 2781218. PMID 22461095.
  66. ^ Evans DA, Clarke CA (September 1961). "Pharmacogenetics". British Medical Bulletin. 17 (3): 234–240. doi:10.1093/oxfordjournals.bmb.a069915. PMID 13697554.
  67. ^ Kalow W (2006). "Pharmacogenetics and pharmacogenomics: origin, status, and the hope for personalized medicine". The Pharmacogenomics Journal. 6 (3): 162–165. doi:10.1038/sj.tpj.6500361. PMID 16415920. S2CID 21761285.
  68. ^ Vogel F (1959). "Moderne probleme der humangenetik" [Modern human genetics problems]. Ergebnisse der Inneren Medizin und Kinderheilkunde [Results of internal medicine and pediatrics] (in German). Berlin, Heidelberg: Springer: 52–125.
  69. ^ Motulsky AG, Qi M (February 2006). "Pharmacogenetics, pharmacogenomics and ecogenetics". Journal of Zhejiang University. Science. B. 7 (2): 169–170. doi:10.1631/jzus.2006.B0169. PMC 1363768. PMID 16421980.
  70. ^ Kalow W (2005). Pharmacogenomics. New York: Taylor & Francis. pp. 552–3. ISBN 978-1-57444-878-8.
  71. ^ Thorpe DS (2001). "Combinatorial chemistry: starting the second decade". The Pharmacogenomics Journal. 1 (4): 229–232. doi:10.1038/sj.tpj.6500045. PMID 11908762. S2CID 1740692.
  72. ^ Paul NW, Fangerau H (December 2006). "Why should we bother? Ethical and social issues in individualized medicine". Current Drug Targets. 7 (12): 1721–1727. doi:10.2174/138945006779025428. PMID 17168846.
  73. ^ Topol E (2012). The Creative Destruction of Medicine: How the Digital Revolution Will Create Better Health Care. New York: Basic Books. ISBN 978-0-465-02550-3.
  74. ^ a b c Corrigan OP (March 2005). "Pharmacogenetics, ethical issues: review of the Nuffield Council on Bioethics Report". Journal of Medical Ethics. 31 (3): 144–148. doi:10.1136/jme.2004.007229. PMC 1734105. PMID 15738433.
  75. ^ Breckenridge A, Lindpaintner K, Lipton P, McLeod H, Rothstein M, Wallace H (September 2004). "Pharmacogenetics: ethical problems and solutions". Nature Reviews. Genetics. 5 (9): 676–680. doi:10.1038/nrg1431. PMID 15372090. S2CID 6149591.
  76. ^ Barh D, Dhawan D, Ganguly NK (2013). Barh D, Dhawan D, Ganguly NK (eds.). Omics for Personalized Medicine. India: Springer Media. doi:10.1007/978-81-322-1184-6. ISBN 978-81-322-1183-9. S2CID 46120003.
  77. ^ Stram D (2014). "Post-GWAS Analyses". Design, Analysis, and Interpretation of Genome-Wide Association Scans. Statistics for Biology and Health. Los Angeles: Springer Science and Business Media. pp. 285–327. doi:10.1007/978-1-4614-9443-0_8. ISBN 978-1-4614-9442-3.
  78. ^ Gaedigk A, Ingelman-Sundberg M, Miller NA, Leeder JS, Whirl-Carrillo M, Klein TE (March 2018). "The Pharmacogene Variation (PharmVar) Consortium: Incorporation of the Human Cytochrome P450 (CYP) Allele Nomenclature Database". Clinical Pharmacology and Therapeutics. 103 (3): 399–401. doi:10.1002/cpt.910. PMC 5836850. PMID 29134625.
  79. ^ Preissner S, Kroll K, Dunkel M, Senger C, Goldsobel G, Kuzman D, et al. (January 2010). "SuperCYP: a comprehensive database on Cytochrome P450 enzymes including a tool for analysis of CYP-drug interactions". Nucleic Acids Research. 38 (Database issue): D237–D243. doi:10.1093/nar/gkp970. PMC 2808967. PMID 19934256.
  80. ^ Thorn CF, Klein TE, Altman RB (2013). "PharmGKB: The Pharmacogenomics Knowledge Base". Pharmacogenomics. Methods in Molecular Biology. Vol. 1015. Clifton, N.J. pp. 311–20. doi:10.1007/978-1-62703-435-7_20. ISBN 978-1-62703-434-0. PMC 4084821. PMID 23824865.{{cite book}}: CS1 maint: location missing publisher (link)
  81. ^ "DBSNP Home Page". National Center for Biotechnology Information, U.S. National Library of Medicine.
  82. ^ Smigielski EM, Sirotkin K, Ward M, Sherry ST (January 2000). "dbSNP: a database of single nucleotide polymorphisms". Nucleic Acids Research. 28 (1): 352–355. doi:10.1093/nar/28.1.352. PMC 102496. PMID 10592272.
  83. ^ Papadopoulos P, Viennas E, Gkantouna V, Pavlidis C, Bartsakoulia M, Ioannou ZM, et al. (January 2014). "Developments in FINDbase worldwide database for clinically relevant genomic variation allele frequencies". Nucleic Acids Research. 42 (Database issue): D1020–D1026. doi:10.1093/nar/gkt1125. PMC 3964978. PMID 24234438.
  84. ^ "Table of Pharmacogenomic Biomarkers in Drug Labeling". FDA. 18 August 2021.
  85. ^ "SNPedia".
  86. ^ Cariaso M, Lennon G (January 2012). "SNPedia: a wiki supporting personal genome annotation, interpretation and analysis". Nucleic Acids Research. 40 (Database issue): D1308–D1312. doi:10.1093/nar/gkr798. PMC 3245045. PMID 22140107.
  87. ^ "Pharmacogenomics Research Network PGRN".

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