Translin

Translin is a DNA-binding protein that in humans is encoded by the TSN gene. Together with translin-associated factor X, translin forms the component 3 of promoter of RISC (C3PO) complex which facilitates endonucleolytic cleavage of the passenger strand during microRNA loading into the RNA-induced silencing complex (RISC).

TSN
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 1J1J, 3PJA, 3QB5, 4WYV
Identifiers
Aliases	TSN, BCLF-1, C3PO, RCHF1, REHF-1, TBRBP, TRSLN, translin
External IDs	OMIM: 600575; MGI: 109263; HomoloGene: 3397; GeneCards: TSN; OMA:TSN - orthologs
Gene location (Human) Chr. Chromosome 2 (human) Band 2q14.3 Start 121,737,103 bp End 121,767,853 bp
Gene location (Mouse) Chr. Chromosome 1 (mouse) Band 1|1 E2.3 Start 118,222,508 bp End 118,239,469 bp
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in gonad secondary oocyte skin of hip optic nerve skin of thigh ganglionic eminence thoracic diaphragm germinal epithelium islet of Langerhans superficial temporal artery Top expressed in trigeminal ganglion endothelial cell of lymphatic vessel Rostral migratory stream renal corpuscle primitive streak medullary collecting duct vestibular sensory epithelium neural tube Paneth cell superior cervical ganglion More reference expression data BioGPS More reference expression data
Gene ontology Molecular function DNA binding nuclease activity endonuclease activity sequence-specific DNA binding protein binding mRNA binding hydrolase activity RNA binding single-stranded DNA binding identical protein binding endoribonuclease activity protein-containing complex binding Cellular component cytoplasm cytosol nucleus Biological process DNA recombination nucleic acid phosphodiester bond hydrolysis RNA metabolic process RNA phosphodiester bond hydrolysis, endonucleolytic production of siRNA involved in RNA interference Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 7247 22099 Ensembl ENSG00000211460 ENSMUSG00000026374 UniProt Q15631 Q62348 RefSeq (mRNA) NM_001261401 NM_004622 NM_011650 RefSeq (protein) NP_001248330 NP_004613 NP_035780 Location (UCSC) Chr 2: 121.74 – 121.77 Mb Chr 1: 118.22 – 118.24 Mb PubMed search
Wikidata
View/Edit Human View/Edit Mouse

Function

This gene encodes a DNA-binding protein which specifically recognizes conserved target sequences at the breakpoint junction of chromosomal translocations. Translin polypeptides form a multimeric structure that is responsible for its DNA-binding activity. Recombination-associated motifs and translin-binding sites are present at recombination hotspots and may serve as indicators of breakpoints in genes which are fused by translocations. These binding activities may play a crucial role in chromosomal translocation in lymphoid neoplasms.

Interactions

Translin has been shown to interact with PPP1R15A.

Further reading

• Aoki K, Suzuki K, Sugano T, Tasaka T, Nakahara K, Kuge O, Omori A, Kasai M (June 1995). "A novel gene, Translin, encodes a recombination hotspot binding protein associated with chromosomal translocations". Nature Genetics. 10 (2): 167–74. doi:10.1038/ng0695-167. PMID 7663511. S2CID 9678546.
• Maruyama K, Sugano S (January 1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides". Gene. 138 (1–2): 171–4. doi:10.1016/0378-1119(94)90802-8. PMID 8125298.
• Aoki K, Nakahara K, Ikegawa C, Seto M, Takahashi T, Minowada J, Strominger JL, Maziarz RT, Kasai M (April 1994). "Nuclear proteins binding to a novel target sequence within the recombination hotspot regions of Bcl-2 and the immunoglobulin DH gene family". Oncogene. 9 (4): 1109–15. PMID 8134113.
• Aoki K, Ishida R, Kasai M (January 1997). "Isolation and characterization of a cDNA encoding a Translin-like protein, TRAX". FEBS Letters. 401 (2–3): 109–12. doi:10.1016/S0014-5793(96)01444-5. PMID 9013868. S2CID 46704829.
• Kasai M, Matsuzaki T, Katayanagi K, Omori A, Maziarz RT, Strominger JL, Aoki K, Suzuki K (April 1997). "The translin ring specifically recognizes DNA ends at recombination hot spots in the human genome". The Journal of Biological Chemistry. 272 (17): 11402–7. doi:10.1074/jbc.272.17.11402. PMID 9111049.
• Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, Suyama A, Sugano S (October 1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149.
• Hasegawa T, Isobe K (August 1999). "Evidence for the interaction between Translin and GADD34 in mammalian cells". Biochimica et Biophysica Acta (BBA) - General Subjects. 1428 (2–3): 161–8. doi:10.1016/s0304-4165(99)00060-4. PMID 10434033.
• Badge RM, Yardley J, Jeffreys AJ, Armour JA (May 2000). "Crossover breakpoint mapping identifies a subtelomeric hotspot for male meiotic recombination". Human Molecular Genetics. 9 (8): 1239–44. doi:10.1093/hmg/9.8.1239. PMID 10767349.
• Hosaka T, Kanoe H, Nakayama T, Murakami H, Yamamoto H, Nakamata T, Tsuboyama T, Oka M, Kasai M, Sasaki MS, Nakamura T, Toguchida J (November 2000). "Translin binds to the sequences adjacent to the breakpoints of the TLS and CHOP genes in liposarcomas with translocation t(12;6)". Oncogene. 19 (50): 5821–5. doi:10.1038/sj.onc.1203943. hdl:2433/150205. PMID 11126370.
• Chennathukuzhi VM, Kurihara Y, Bray JD, Hecht NB (April 2001). "Trax (translin-associated factor X), a primarily cytoplasmic protein, inhibits the binding of TB-RBP (translin) to RNA". The Journal of Biological Chemistry. 276 (16): 13256–63. doi:10.1074/jbc.M009707200. PMID 11278549.
• Lee SP, Fuior E, Lewis MS, Han MK (November 2001). "Analytical ultracentrifugation studies of translin: analysis of protein-DNA interactions using a single-stranded fluorogenic oligonucleotide". Biochemistry. 40 (46): 14081–8. doi:10.1021/bi010302t. PMID 11705401.
• Erdemir T, Bilican B, Oncel D, Goding CR, Yavuzer U (January 2002). "DNA damage-dependent interaction of the nuclear matrix protein C1D with Translin-associated factor X (TRAX)". Journal of Cell Science. 115 (Pt 1): 207–16. doi:10.1242/jcs.115.1.207. hdl:11693/24740. PMID 11801738.
• Han MK, Lin P, Paek D, Harvey JJ, Fuior E, Knutson JR (March 2002). "Fluorescence studies of pyrene maleimide-labeled translin: excimer fluorescence indicates subunits associate in a tail-to-tail configuration to form octamer". Biochemistry. 41 (10): 3468–76. doi:10.1021/bi015901e. PMID 11876655.
• Sengupta K, Rao BJ (December 2002). "Translin binding to DNA: recruitment through DNA ends and consequent conformational transitions". Biochemistry. 41 (51): 15315–26. doi:10.1021/bi026378m. PMID 12484770.
• Li K, Wang L, Cheng J, Lu YY, Zhang LX, Mu JS, Hong Y, Liu Y, Duan HJ, Wang G, Li L, Chen JM (February 2003). "Interaction between hepatitis C virus core protein and translin protein--a possible molecular mechanism for hepatocellular carcinoma and lymphoma caused by hepatitis C virus". World Journal of Gastroenterology. 9 (2): 300–3. doi:10.3748/wjg.v9.i2.300. PMC 4611333. PMID 12532453.
• Yang S, Cho YS, Chennathukuzhi VM, Underkoffler LA, Loomes K, Hecht NB (March 2004). "Translin-associated factor X is post-transcriptionally regulated by its partner protein TB-RBP, and both are essential for normal cell proliferation". The Journal of Biological Chemistry. 279 (13): 12605–14. doi:10.1074/jbc.M313133200. PMID 14711818.

External links

• Overview of all the structural information available in the PDB for UniProt: Q15631 (Human Translin) at the PDBe-KB.
• Overview of all the structural information available in the PDB for UniProt: Q62348 (Mouse Translin) at the PDBe-KB.