Talk:Artificial gene synthesis

Removed a sentence I couldn't understand

I couldn't figure out what was meant by "Currently available libraries of synthetic genes undergone gene synthesis process consists of dozens of thouthands elements." I removed it, but the article definitely lost some information in the process. Iknowyourider (t c) 07:54, 23 June 2007 (UTC)

It is OK to remove that. Sorry for being unclear. I was actually referring to libraries like this one: http://www.dna20.com/planetgene.php BTW, thanx for clean up! TestPilot 18:20, 23 June 2007 (UTC)

Requested move

To synthetic DNA

The following discussion is an archived discussion of a requested move. Please do not modify it. Subsequent comments should be made in a new section on the talk page. No further edits should be made to this section.

The result of the move request was: page not moved, but see discussion below. Regards, Arbitrarily0 ^(talk) 15:45, 30 May 2010 (UTC)

---

Gene synthesis → Synthetic DNA — The topic of this article isn't necessarily about entire genes, but always about DNA. Gene synthesis doesn't distinguish from natural DNA replication. Furthermore, synthetic DNA expands the scope beyond the synthesis itself (basically already described in oligonucleotide synthesis), potentially including applications of the product as well. Mikael Häggström (talk) 05:52, 23 May 2010 (UTC)

Not sure. Synthetic DNA sounds too similar to oligonucleotide synthesis. We should see what is the preferred name in the literature for this kind of technology (I don't know: I am a protein biophysics guy :) ). If there is none, perhaps Artificial gene synthesis could clarify that we exclude natural processes? --Cyclopia^talk 13:49, 25 May 2010 (UTC)

I agree with Cyclopia's suggestions. Mikael, you mentioned at MCB talk you want to make some changes, and I think the nature of those changes is relevant to any proposed rename. The current article does seem to focus on the synthesis of genes specifically (or genomes), as opposed to merely discussing the technical aspects of constructing DNA, but I don't oppose expanding the scope and renaming accordingly. Adrian J. Hunter^{(talk•contribs)} 15:13, 25 May 2010 (UTC)

I oppose the move. I agree with Cyclopia that the title "Synthetic DNA" would include oligonucleotide synthesis in its scope as well, whereas the current article does not, except as a summary section. I don't think that "Gene synthesis" could be mistaken to be about natural DNA replication, but if so I think a hatnote would solve that. Antony-22 (talk) 17:07, 25 May 2010 (UTC)

Well, perhaps artificial gene synthesis would also work, but it would still not be the article title for where one really expects to find facts about synthetic entire genomes or applications of the products. Synthetic DNA could be an umbrella article that introduces Oligonucleotide synthesis as well. Mikael Häggström (talk) 04:38, 26 May 2010 (UTC)

If we want to talk about artificial genome synthesis, we can have a separate article. Or we can have a redirect to a section in "artificial gene synthesis". Either way, I see no real problem. --Cyclopia^talk 23:11, 27 May 2010 (UTC)

The above discussion is preserved as an archive of a requested move. Please do not modify it. Subsequent comments should be made in a new section on this talk page. No further edits should be made to this section.

To artificial gene synthesis

The following discussion is an archived discussion of the proposal. Please do not modify it. Subsequent comments should be made in a new section on the talk page. No further edits should be made to this section.

Page moved'. Vegaswikian (talk) 07:04, 5 June 2010 (UTC)

Gene synthesis → artificial gene synthesis — All right, a move to artificial gene synthesis would be fair enough. Applications of the product may still be located in this article.Mikael Häggström (talk) 07:15, 29 May 2010 (UTC)

• Support per discussion above. --Cyclopia^talk 13:06, 30 May 2010 (UTC)

The above discussion is preserved as an archive of the proposal. Please do not modify it. Subsequent comments should be made in a new section on this talk page. No further edits should be made to this section.

amino acid designated 'U'

"The letter V was used since there is no amino acid designated by letter U"
There is an amino-acid designated U: Selenocysteine. It is included in proteins in a template-directed manner, but there additional structural conditions for requirement.
Speculation as to why this wasn't used by the Venter institute would of course be original research, but could we update the article to reflect this? At the moment it's in error.Loris (talk) 17:10, 22 July 2010 (UTC)

Well spotted – a subtle point that was overlooked by the source (Wired). I've tweaked the text to avoid the error. Feel free to be bold and edit as you see fit. Adrian J. Hunter^{(talk•contribs)} 12:22, 23 July 2010 (UTC)

German Wiki

I used the version from 21th April of this Article for translation into the German Wikipedia. Thanks to everyone who has worked on this so far, I hope you don't mind.--LacZ (talk) 20:14, 27 May 2013 (UTC)

Cost and wording

I'm surprise that this article would claim that this is "often more economical than classical cloning and mutagenesis procedures." Seems like it's something written by someone who doesn't know how to do cloning and mutagenesis work efficiently and cheaply. Artificial synthesis of a gene not based on traditional molecular cloning methods can only be cheaper on certain procedures, for most standard cloning and mutagenesis work it is still not yet cheaper (it may become cheaper in a few years' time, but for now not yet). Note also that traditional methods such as PCR can also be used to synthesize gene artificially, therefore the title of the article is misleading when it refers only to solid-phase DNA synthesis. Hzh (talk) 10:12, 11 October 2016 (UTC)

External links modified

Hello fellow Wikipedians,

I have just modified 2 external links on Artificial gene synthesis. Please take a moment to review my edit. If you have any questions, or need the bot to ignore the links, or the page altogether, please visit this simple FaQ for additional information. I made the following changes:

• Corrected formatting/usage for http://www.genengnews.com/gen-articles/new-tools-facilitate-protein-expression/3273/
• Added archive https://web.archive.org/web/20140509001048/http://www.utsandiego.com/news/2014/may/08/tp-life-engineered-with-expanded-genetic-code/ to http://www.utsandiego.com/news/2014/may/08/tp-life-engineered-with-expanded-genetic-code/

When you have finished reviewing my changes, you may follow the instructions on the template below to fix any issues with the URLs.

This message was posted before February 2018. After February 2018, "External links modified" talk page sections are no longer generated or monitored by InternetArchiveBot. No special action is required regarding these talk page notices, other than regular verification using the archive tool instructions below. Editors have permission to delete these "External links modified" talk page sections if they want to de-clutter talk pages, but see the RfC before doing mass systematic removals. This message is updated dynamically through the template {{source check}} (last update: 5 June 2024).

• If you have discovered URLs which were erroneously considered dead by the bot, you can report them with this tool.
• If you found an error with any archives or the URLs themselves, you can fix them with this tool.

Cheers.—InternetArchiveBot (Report bug) 00:36, 10 July 2017 (UTC)

Editing for a school project

Hi all,

I am planning to edit this page to more accurately reflect the different stages of artificial gene synthesis - the current article focuses mainly on oligonucleotide assembly methods, but there is little mention of DNA assembly methods on how to assemble gene constructs from oligonucleotides. I have gone through several other pages, e.g. synthetic genomics, molecular cloning, but those articles also do not make significant mention of DNA assembly methods. There are standalone pages talking about individual methods, e.g. Gibson assembly or BioBricks, but the current state of research in this field has expanded significantly beyond these two methods, and there appears to be a gap in reflecting this on Wikipedia articles related to synthetic biology.Scarecrow399 (talk) 18:24, 12 March 2020 (UTC)