The Mistress can refer to:
Film and television
edit- The Mistress (TV series), a British sitcom
- The Mistress (1927 film), a silent German film
- The Mistress (1962 film), a 1962 Swedish film
- The Mistress (2012 film), a 2012 Filipino film
Doctor Who characters
edit- Missy (Doctor Who), a female incarnation of the Master character in Doctor Who
- The Mistress, a depiction of Romana II in a series of Doctor Who audioplays