Toughie or Toughy may refer to:
- Toughie (frog), the last surviving member of the species Rabbs' fringe-limbed treefrog
- Toughie Brasuhn (1923-1971), American roller derby skater
- Toughy, a character in Grasshopper Island, a 1971 British children's television serial
- Toughy, a character in Martin Morning, a 21st century French animated television series
- Toughy, a DC Comics character