Unique molecular identifiers (UMIs), or molecular barcodes (MBC) are short sequences or molecular "tags" added to DNA fragments in some next generation sequencing library preparation protocols to identify the input DNA molecule. These tags are added before PCR amplification, and can be used to reduce errors and quantitative bias introduced by the amplification.
Applications include analysis of unique cDNAs to avoid PCR biases in iCLIP,[1] variant calling in ctDNA, gene expression in single-cell RNA-seq (scRNA-seq)[2][3] and haplotyping via linked reads[clarification needed].
See also
editReferences
edit- ^ König, Julian; Zarnack, Kathi; Rot, Gregor; Curk, Tomaz; Kayikci, Melis; Zupan, Blaz; Turner, Daniel J.; Luscombe, Nicholas M.; Ule, Jernej (July 2010). "iCLIP reveals the function of hnRNP particles in splicing at individual nucleotide resolution". Nature Structural & Molecular Biology. 17 (7): 909–915. doi:10.1038/nsmb.1838. ISSN 1545-9985. PMC 3000544. PMID 20601959.
- ^ Kivioja T, Vähärautio A, Karlsson K, Bonke M, Enge M, Linnarsson S, Taipale J (2012). "Counting absolute numbers of molecules using unique molecular identifiers". Nat. Methods. 9 (1): 72–4. doi:10.1038/nmeth.1778. PMID 22101854. S2CID 39225091.
- ^ Islam S, Zeisel A, Joost S, La Manno G, Zajac P, Kasper M, Lönnerberg P, Linnarsson S (2014). "Quantitative single-cell RNA-seq with unique molecular identifiers". Nat. Methods. 11 (2): 163–6. doi:10.1038/nmeth.2772. PMID 24363023. S2CID 6765530.
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