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Ethidium Bromide is also used during DNA fragment separation by agarose gel electrophoresis. It is added to running buffer and binds by intercalating between DNA base pairs. When the agarose gel is illuminated using UV light, DNA bands become visible. Intercalation of EtBr can alter properties of the DNA molecule, such as charge, weight, conformation, and flexibility. Since the mobilities of DNA molecules through the agarose gel are measured relative to a molecular weight standard, the effects of EtBr can be critical to determining the sizes of molecules.[1]
Sigmon, J. and Larcom, L. L. (1996), The effect of ethidium bromide on mobility of DNA fragments in agarose gel electrophoresis. ELECTROPHORESIS, 17: 1524–1527. doi:10.1002/elps.1150171003
| This is a user sandbox of Ose347. You can use it for testing or practicing edits. This is not the sandbox where you should draft your assigned article for a dashboard.wikiedu.org course. To find the right sandbox for your assignment, visit your Dashboard course page and follow the Sandbox Draft link for your assigned article in the My Articles section. |
- ^ Sigmon, J.; Larcom, L. L. (October 1996). "The effect of ethidium bromide on mobility of DNA fragments in agarose gel electrophoresis". Electrophoresis. 17 (10): 1524–1527. doi:10.1002/elps.1150171003. ISSN 0173-0835. PMID 8957173.