Wayne Osborne may refer to:
- Wayne Osborne (baseball) (1912–1987), American baseball player
- Wayne Osborne (footballer) (born 1977), English former professional footballer
- Wayne Osborne (Biologist) (born 1964), Automotive Service Specialist, Biologist, Educator. Researcher for Turtle Survival Alliance- North American Freshwater Turtle Research Group
- Wayne Osborne (born 1960), head of Koop, Larry Page’s family office, since 2012.