2'-hydroxybiphenyl-2-sulfinate desulfinase

In enzymology, a 2'-hydroxybiphenyl-2-sulfinate desulfinase (EC 3.13.1.3) is an enzyme that catalyzes the chemical reaction

2-(2-hydroxyphenyl)benzenesulfinate hydrolase
Identifiers
EC no.	3.13.1.3
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Gene Ontology	AmiGO / QuickGO
Search PMC articles PubMed articles NCBI proteins

2'-hydroxybiphenyl-2-sulfinate + H₂O $\rightleftharpoons$ 2-hydroxybiphenyl + sulfite

Thus, the two substrates of this enzyme are 2'-hydroxybiphenyl-2-sulfinate and H₂O, whereas its two products are 2-hydroxybiphenyl and sulfite.

This enzyme belongs to the family of hydrolases, specifically those acting on carbon-sulfur bonds. The systematic name of this enzyme class is 2'-hydroxybiphenyl-2-sulfinate sulfohydrolase. Other names in common use include gene dszB-encoded hydrolase, 2-(2-hydroxyphenyl) benzenesulfinate:H₂O hydrolase, DszB, HBPSi desulfinase, 2-(2-hydroxyphenyl) benzenesulfinate sulfohydrolase, HPBS desulfinase, 2-(2-hydroxyphenyl)benzenesulfinate hydrolase, 2-(2'-hydroxyphenyl)benzenesulfinate desulfinase, and 2-(2-hydroxyphenyl)benzenesulfinate desulfinase.

Structural studies

As of late 2007, 3 structures have been solved for this class of enzymes, with PDB accession codes 2DE2, 2DE3, and 2DE4.

References

• Oldfield C, Pogrebinsky O, Simmonds J, Olson ES, Kulpa CF (September 1997). "Elucidation of the metabolic pathway for dibenzothiophene desulphurization by Rhodococcus sp. strain IGTS8 (ATCC 53968)". Microbiology. 143 (9): 2961–73. doi:10.1099/00221287-143-9-2961. PMID 9308179.
• Nakayama N, Matsubara T, Ohshiro T, Moroto Y, Kawata Y, Koizumi K, Hirakawa Y, Suzuki M, Maruhashi K, Izumi Y, Kurane R (July 2002). "A novel enzyme, 2'-hydroxybiphenyl-2-sulfinate desulfinase (DszB), from a dibenzothiophene-desulfurizing bacterium Rhodococcus erythropolis KA2-5-1: gene overexpression and enzyme characterization". Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics. 1598 (1–2): 122–30. doi:10.1016/s0167-4838(02)00365-5. PMID 12147352.
• Watkins LM, Rodriguez R, Schneider D, Broderick R, Cruz M, Chambers R, Ruckman E, Cody M, Mrachko GT (July 2003). "Purification and characterization of the aromatic desulfinase, 2-(2'-hydroxyphenyl)benzenesulfinate desulfinase". Archives of Biochemistry and Biophysics. 415 (1): 14–23. doi:10.1016/S0003-9861(03)00230-3. PMID 12801508.