23S rRNA (guanine748-N1)-methyltransferase (EC 2.1.1.188, Rlma(II), Rlma2, 23S rRNA m1G748 methyltransferase, RlmaII, Rlma II, tylosin-resistance methyltransferase RlmA(II), TlrB, rRNA large subunit methyltransferase II) is an enzyme with systematic name S-adenosyl-L-methionine:23S rRNA (guanine748-N1)-methyltransferase.[1][2][3][4][5][6] This enzyme catalyses the following chemical reaction
| 23S rRNA (guanine748-N1)-methyltransferase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC no. | 2.1.1.188 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| |||||||||
- S-adenosyl-L-methionine + guanine748 in 23S rRNA S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
The enzyme specifically methylates guanine748 at N1 in 23S rRNA.
References
edit- ^ Douthwaite S, Crain PF, Liu M, Poehlsgaard J (April 2004). "The tylosin-resistance methyltransferase RlmA(II) (TlrB) modifies the N-1 position of 23S rRNA nucleotide G748". Journal of Molecular Biology. 337 (5): 1073–7. doi:10.1016/j.jmb.2004.02.030. PMID 15046978.
- ^ Liu M, Kirpekar F, Van Wezel GP, Douthwaite S (August 2000). "The tylosin resistance gene tlrB of Streptomyces fradiae encodes a methyltransferase that targets G748 in 23S rRNA". Molecular Microbiology. 37 (4): 811–20. doi:10.1046/j.1365-2958.2000.02046.x. hdl:1887/3221371. PMID 10972803.
- ^ Lebars I, Yoshizawa S, Stenholm AR, Guittet E, Douthwaite S, Fourmy D (January 2003). "Structure of 23S rRNA hairpin 35 and its interaction with the tylosin-resistance methyltransferase RlmAII". The EMBO Journal. 22 (2): 183–92. doi:10.1093/emboj/cdg022. PMC 140097. PMID 12514124.
- ^ Lebars I, Husson C, Yoshizawa S, Douthwaite S, Fourmy D (September 2007). "Recognition elements in rRNA for the tylosin resistance methyltransferase RlmA(II)". Journal of Molecular Biology. 372 (2): 525–34. doi:10.1016/j.jmb.2007.06.068. PMID 17673230.
- ^ Douthwaite S, Jakobsen L, Yoshizawa S, Fourmy D (May 2008). "Interaction of the tylosin-resistance methyltransferase RlmA II at its rRNA target differs from the orthologue RlmA I". Journal of Molecular Biology. 378 (5): 969–75. doi:10.1016/j.jmb.2008.03.024. PMID 18406425.
- ^ Liu M, Douthwaite S (April 2002). "Methylation at nucleotide G745 or G748 in 23S rRNA distinguishes Gram-negative from Gram-positive bacteria". Molecular Microbiology. 44 (1): 195–204. doi:10.1046/j.1365-2958.2002.02866.x. PMID 11967079.
External links
edit- 23S+rRNA+(guanine748-N1)-methyltransferase at the U.S. National Library of Medicine Medical Subject Headings (MeSH)
