3-hydroxybenzoate 4-monooxygenase

In enzymology, a 3-hydroxybenzoate 4-monooxygenase (EC 1.14.13.23) is an enzyme that catalyzes the chemical reaction

3-hydroxybenzoate 4-monooxygenase
Identifiers
EC no.1.14.13.23
CAS no.37256-76-1
Databases
IntEnzIntEnz view
BRENDABRENDA entry
ExPASyNiceZyme view
KEGGKEGG entry
MetaCycmetabolic pathway
PRIAMprofile
PDB structuresRCSB PDB PDBe PDBsum
Gene OntologyAmiGO / QuickGO
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PMCarticles
PubMedarticles
NCBIproteins
3-hydroxybenzoate + NADPH + H+ + O2 3,4-dihydroxybenzoate + NADP+ + H2O

The 4 substrates of this enzyme are 3-hydroxybenzoate, NADPH, H+, and O2, whereas its 3 products are 3,4-dihydroxybenzoate, NADP+, and H2O.

This enzyme belongs to the family of oxidoreductases, specifically those acting on paired donors, with O2 as oxidant and incorporation or reduction of oxygen. The oxygen incorporated need not be derived from O2 with NADH or NADPH as one donor, and incorporation of one atom o oxygen into the other donor. The systematic name of this enzyme class is 3-hydroxybenzoate,NADPH:oxygen oxidoreductase (4-hydroxylating). This enzyme is also called 3-hydroxybenzoate 4-hydroxylase. This enzyme participates in benzoate degradation via hydroxylation and 2,4-dichlorobenzoate degradation. It employs one cofactor, FAD.

Structural studies

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As of late 2007, two structures have been solved for this class of enzymes, with PDB accession codes 2DKH and 2DKI.

References

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  • Michalover JL, Ribbons DW, Hughes H (1973). "3-Hydroxybenzoate 4-hydroxylase from Pseudomonas testosteroni". Biochem. Biophys. Res. Commun. 55 (3): 888–96. doi:10.1016/0006-291X(73)91227-8. PMID 4148586.
  • Premkumar R, Rao PV, Sreeleela NS, Vaidyanathan CS (1969). "m-Hydroxybenzoic acid 4-hydroxylase from Aspergillus niger". Can. J. Biochem. 47 (8): 825–7. doi:10.1139/o69-129. PMID 4390252.