53rd Brigade or 53rd Infantry Brigade may refer to:
- 53rd Brigade (Greece) of the Greek Army during the Greek Civil War
- 53rd Indian Brigade of the British Indian Army in the First World War
- 53rd Indian Infantry Brigade of the British Indian Army in the Second World War
- 53rd Brigade (United Kingdom) of the British Army during the First and Second World Wars
- 53rd Mountain Motorized Infantry Brigade (People's Republic of China)
- 53rd Anti-Aircraft Rocket Brigade of the Russian Ground Forces
- 53rd Mechanized Brigade (Ukraine)
- 53rd Infantry Brigade Combat Team (United States)