53rd Regiment or 53rd Infantry Regiment may refer to:
- 53rd (Napier's) Regiment of Foot, a unit of the British Army
- 53rd (Shropshire) Regiment of Foot, a unit of the British Army
- 53rd Sikhs (Frontier Force), a unit of the British Indian Army
- 53rd (City of London) Heavy Anti-Aircraft Regiment, Royal Artillery
- 53rd Anti-Aircraft Missile Regiment (Romania)
- 53rd Coast Artillery Regiment, a unit of the United States Army
- 53rd Infantry Regiment (Imperial Japanese Army)
- 53rd Infantry Regiment (United States)
- 53rd Coast Artillery Regiment, United States
American Civil War regiments:
- 53rd Regiment Alabama Cavalry, a unit of the Confederate States Army
- 53rd Illinois Infantry Regiment, a unit of the Union Army
- 53rd Indiana Infantry Regiment, a unit of the Union Army
- 53rd Regiment Kentucky Volunteer Mounted Infantry, a unit of the Union Army
- 53rd Regiment Massachusetts Volunteer Infantry, a unit of the Union Army
- 53rd Ohio Infantry, a unit of the Union Army
- 53rd Pennsylvania Infantry Regiment, a unit of the Union Army
- 53rd Wisconsin Volunteer Infantry Regiment, a unit of the Union Army