Acuff is a surname. Notable people with the surname include:
- Amy Acuff (born 1975), American athlete
- Darius Acuff (born 2006), American basketball player
- Eddie Acuff (1903–1956), American actor
- Roy Acuff (1903–1992), American musician
- Ruth Acuff, American singer-songwriter
See also
edit- Acuff, Texas
- Acuff-Rose Music, American publishing firm