Alternan is a branched α-d-glucan produced by microorganisms. This polymer was first discovered in the bacterium Leuconostoc mesenteroides, which has since been genetically engineered to produce alternan. They genetically engineered the genes for alternansucrase, the enzyme that produces alternan, to get a pure sample. This gene of the enzyme alternansucrase had also been genetically engineered into E. coli.[1]
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| Identifiers | |
|---|---|
| Properties | |
| (C6H10O5)n | |
| Highly soluble | |
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
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This compound is highly soluble in water and has a low viscosity. These characteristics make it a prime candidate to use in the food and cosmetic industries.
Biosynthesis
editAlternan is an exopolysaccharide generated from the glucose monosaccharides of sucrose by the enzyme alternansucrase.[1] The polysaccharide is assembled with α-(1→6) and α-(1→3) glycosidic bonds.[2] Alternansucrase was initially found in Leuconostoc mesenteroides, a bacterial species used in the dairy industry.[3] L. mesenteroides is a gram postitive bacterium that performs fermentation. During fermentation the bacterium produces a very high amount of alternan and dextran, requiring a medium containing sucrose, nitrogen, glutamate, thiamine, and valine. The alternan is then extracted from the fermentation product through centrifugation or filtration.
Industrial use
editAlternan has numerous industrial uses. One of the valuable uses is in food production as an essential component of the sweetener sucromalt, also known as Xtend, which is commonly used in diabetic foods due to its low glycemic index.[4][5] Alternan can also be used to add texture to food or cosmetics in place of fat or oil. [6]
Alternan has shown potential as a bulking agent as it has a low viscosity and high solubility. These properties are shared with other commonly used food extenders and binders, suggesting a promising alternan application.[7]
References
edit- ^ a b Handbook of Hydrocolloids. Elsevier. 2021. doi:10.1016/c2018-0-04245-0. ISBN 978-0-12-820104-6.
- ^ Leathers, Timothy D.; Nunnally, Melinda S.; Côté, Gregory L. (2010-07-07). "Optimization of process conditions for enzymatic modification of alternan using dextranase from Chaetomium erraticum". Carbohydrate Polymers. 81 (3): 732–736. doi:10.1016/j.carbpol.2010.03.030. ISSN 0144-8617.
- ^ Hassan, A. N.; Frank, J. F. (2011-01-01), Fuquay, John W. (ed.), "MICROORGANISMS ASSOCIATED WITH MILK", Encyclopedia of Dairy Sciences (Second Edition), San Diego: Academic Press, pp. 447–457, doi:10.1016/b978-0-12-374407-4.00309-5, ISBN 978-0-12-374407-4, retrieved 2024-10-04
- ^ "Xtend ® Sucromalt". Cargill. Cargill Incorporated. Retrieved 23 October 2024.
- ^ Rich, Joseph. "Lactic Acid Bateria - Friend or Foe? Lactic Acid Bacteria in the Production of Polysaccharides and Fuel Ethanol". Agricultural Research Service U.S. DEPARTMENT OF AGRICULTURE. US Government. Retrieved 3 October 2024.
- ^ "Unified Patents - Analytics Portal". portal.unifiedpatents.com. Retrieved 2024-10-07.
- ^ "Publication : USDA ARS". www.ars.usda.gov. Retrieved 2024-10-23.
- Netrusov, Liyaskina, Kurgaeva, Liyaskina, Yang, Revin, AI, EV, IV, AU, G, VV. "Exopolysaccharides Producing Bacteria: A Review". MDPI. Microorganisms. Retrieved 3 October 2024.
{{cite web}}: CS1 maint: multiple names: authors list (link) - Cifuente, Javier; Colleoni, Christophe; Kalscheuer, Rainer; Guerin, Marcelo. "Architecture, Function, Regulation, and Evolution of α-Glucans Metabolic Enzymes in Prokaryotes". National Library of Medicine. PubMed Central. Retrieved 3 October 2024.