Anguissola is a surname. Notable people with the surname include:
- Caterina Anguissola (c.1508 - 1550), Italian noblewoman
- Elena Anguissola (c. 1532–1584), Italian painter and nun
- Lucia Anguissola (1536/1538–c. 1565–1568), Italian painter
- Sofonisba Anguissola (c. 1532–1625), Italian painter