Anstee is a surname, and may refer to
- Margaret Anstee (1926–2016), United Nations Under-Secretary General (in 1987)
- Nick Anstee (born 1958), 682nd Lord Mayor of the City of London, from 2009 to 2010
- Rae Anstee (born 1932), Australian nurse
Anstee is a surname, and may refer to