BRF1 (gene)

Transcription factor IIIB 90 kDa subunit is a protein that in humans is encoded by the BRF1 gene.

BRF1
Identifiers
Aliases	BRF1, BRF, BRF-1, GTF3B, HEL-S-76p, TAF3B2, TAF3C, TAFIII90, TF3B90, TFIIIB90, hBRF, CFDS, RNA polymerase III transcription initiation factor 90 kDa subunit, RNA polymerase III transcription initiation factor subunit, BRF1 RNA polymerase III transcription initiation factor subunit
External IDs	OMIM: 604902; MGI: 1919558; HomoloGene: 1161; GeneCards: BRF1; OMA:BRF1 - orthologs
Gene location (Human) Chr. Chromosome 14 (human) Band 14q32.33 Start 105,209,286 bp End 105,315,589 bp
Gene location (Mouse) Chr. Chromosome 12 (mouse) Band 12|12 F1 Start 112,923,705 bp End 112,964,324 bp
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in sural nerve right uterine tube right hemisphere of cerebellum C1 segment anterior pituitary right frontal lobe ventricular zone ganglionic eminence apex of heart granulocyte Top expressed in interventricular septum neural layer of retina lens thymus granulocyte lip otolith organ utricle spermatocyte hand More reference expression data BioGPS More reference expression data
Gene ontology Molecular function TBP-class protein binding metal ion binding RNA polymerase III general transcription initiation factor activity RNA polymerase III type 3 promoter sequence-specific DNA binding transcription factor binding protein binding translation initiation factor activity Cellular component transcription factor TFIIIB complex nucleus nucleoplasm Biological process rRNA transcription tRNA transcription regulation of transcription, DNA-templated transcription, DNA-templated positive regulation of transcription by RNA polymerase III transcription by RNA polymerase III transcription initiation from RNA polymerase III promoter transcription preinitiation complex assembly DNA-templated transcription, initiation RNA polymerase III preinitiation complex assembly regulation of transcription by RNA polymerase III translational initiation Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 2972 72308 Ensembl ENSG00000185024 ENSMUSG00000011158 UniProt Q92994 Q8CFK2 RefSeq (mRNA) NM_001242786 NM_001242787 NM_001242788 NM_001242789 NM_001242790 NM_001519 NM_145685 NM_145696 NM_028193 RefSeq (protein) NP_001229715 NP_001229716 NP_001229717 NP_001229718 NP_001229719 NP_001510 NP_663718 NP_663718.1 NP_082469 Location (UCSC) Chr 14: 105.21 – 105.32 Mb Chr 12: 112.92 – 112.96 Mb PubMed search
Wikidata
View/Edit Human View/Edit Mouse

Function

This gene encodes one of the three subunits of the RNA polymerase III transcription factor complex. This complex plays a central role in transcription initiation by RNA polymerase III on genes encoding tRNA, 5S rRNA, and other small structural RNAs. The gene product belongs to the TF2B family. Three alternatively spliced variants encoding three different isoforms, that function at different promoters transcribed by RNA polymerase III, have been identified. A transcript encoding a fourth isoform has not yet been completely characterized.

Interactions

BRF1 (gene) has been shown to interact with:

• RB1,
• RBL1,
• RBL2, and
• TBP

Further reading

• Chesnokov I, Chu WM, Botchan MR, Schmid CW (1997). "p53 inhibits RNA polymerase III-directed transcription in a promoter-dependent manner". Mol. Cell. Biol. 16 (12): 7084–8. doi:10.1128/MCB.16.12.7084. PMC 231711. PMID 8943363.
• Chu WM, Wang Z, Roeder RG, Schmid CW (1997). "RNA polymerase III transcription repressed by Rb through its interactions with TFIIIB and TFIIIC2". J. Biol. Chem. 272 (23): 14755–61. doi:10.1074/jbc.272.23.14755. PMID 9169441.
• Wang Z, Roeder RG (1997). "Three human RNA polymerase III-specific subunits form a subcomplex with a selective function in specific transcription initiation". Genes Dev. 11 (10): 1315–26. doi:10.1101/gad.11.10.1315. PMID 9171375.
• Sutcliffe JE, Cairns CA, McLees A, Allison SJ, Tosh K, White RJ (June 1999). "RNA polymerase III transcription factor IIIB is a target for repression by pocket proteins p107 and p130". Mol. Cell. Biol. 19 (6): 4255–61. doi:10.1128/mcb.19.6.4255. PMC 104385. PMID 10330166.
• Hsieh YJ, Wang Z, Kovelman R, Roeder RG (1999). "Cloning and characterization of two evolutionarily conserved subunits (TFIIIC102 and TFIIIC63) of human TFIIIC and their involvement in functional interactions with TFIIIB and RNA polymerase III". Mol. Cell. Biol. 19 (7): 4944–52. doi:10.1128/MCB.19.7.4944. PMC 84305. PMID 10373544.
• Hsieh YJ, Kundu TK, Wang Z, Kovelman R, Roeder RG (November 1999). "The TFIIIC90 subunit of TFIIIC interacts with multiple components of the RNA polymerase III machinery and contains a histone-specific acetyltransferase activity". Mol. Cell. Biol. 19 (11): 7697–704. doi:10.1128/mcb.19.11.7697. PMC 84812. PMID 10523658.
• McCulloch V, Hardin P, Peng W, Ruppert JM, Lobo-Ruppert SM (August 2000). "Alternatively spliced hBRF variants function at different RNA polymerase III promoters". EMBO J. 19 (15): 4134–43. doi:10.1093/emboj/19.15.4134. PMC 306597. PMID 10921893.
• Johnston IM, Allison SJ, Morton JP, Schramm L, Scott PH, White RJ (June 2002). "CK2 forms a stable complex with TFIIIB and activates RNA polymerase III transcription in human cells". Mol. Cell. Biol. 22 (11): 3757–68. doi:10.1128/MCB.22.11.3757-3768.2002. PMC 133823. PMID 11997511.
• Cabart P, Murphy S (2002). "Assembly of human small nuclear RNA gene-specific transcription factor IIIB complex de novo on and off promoter". J. Biol. Chem. 277 (30): 26831–8. doi:10.1074/jbc.M203119200. PMID 12016223.
• Willis IM (2002). "A universal nomenclature for subunits of the RNA polymerase III transcription initiation factor TFIIIB". Genes Dev. 16 (11): 1337–8. doi:10.1101/gad.998102. PMID 12066800.
• Stoecklin G, Colombi M, Raineri I, Leuenberger S, Mallaun M, Schmidlin M, Gross B, Lu M, Kitamura T, Moroni C (September 2002). "Functional cloning of BRF1, a regulator of ARE-dependent mRNA turnover". EMBO J. 21 (17): 4709–18. doi:10.1093/emboj/cdf444. PMC 126184. PMID 12198173.
• Weser S, Riemann J, Seifart KH, Meissner W (2003). "Assembly and isolation of intermediate steps of transcription complexes formed on the human 5S rRNA gene". Nucleic Acids Res. 31 (9): 2408–16. doi:10.1093/nar/gkg345. PMC 154231. PMID 12711686.
• Schmidlin M, Lu M, Leuenberger SA, Stoecklin G, Mallaun M, Gross B, Gherzi R, Hess D, Hemmings BA, Moroni C (December 2004). "The ARE-dependent mRNA-destabilizing activity of BRF1 is regulated by protein kinase B". EMBO J. 23 (24): 4760–9. doi:10.1038/sj.emboj.7600477. PMC 535089. PMID 15538381.

External links

• FactorBook BRF1
• Human BRF1 genome location and BRF1 gene details page in the UCSC Genome Browser.