Benacerraf (Hebrew: בנאסרף Ben-Aseraf) is a surname. Notable people with the surname include:
- Baruj Benacerraf (1920–2011), Venezuelan immunologist
- Beryl Benacerraf (1949–2022), American radiologist and obstetrics researcher
- Margot Benacerraf (1926–2024), Venezuelan film director
- Paul Benacerraf (born 1931), French-American philosopher