In enzymology, a benzene 1,2-dioxygenase (EC 1.14.12.3) is an enzyme that catalyzes the chemical reaction
| benzene 1,2-dioxygenase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC no. | 1.14.12.3 | ||||||||
| CAS no. | 9075-66-5 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| Gene Ontology | AmiGO / QuickGO | ||||||||
| |||||||||
The 4 substrates of this enzyme are benzene, NADH, H+, and O2, whereas its two products are cis-cyclohexa-3,5-diene-1,2-diol and NAD+.
Classification
editThis enzyme belongs to the family of oxidoreductases, specifically those acting on paired donors, with O2 as oxidant and incorporation or reduction of oxygen. The oxygen incorporated need not be derived from O2 with NADH or NADPH as one donor, and incorporation of two atoms of oxygen into the other donor.
Nomenclature
editThe systematic name of this enzyme class is benzene,NADH:oxygen oxidoreductase (1,2-hydroxylating). Other names in common use include benzene hydroxylase, and benzene dioxygenase.
Biological role
editThis enzyme participates in naphthalene and anthracene degradation. It has 4 cofactors: FAD, Iron, Sulfur, and Iron-sulfur.
References
edit- Gibson DT, Koch JR, Kallio RE (1968). "Oxidative degradation of aromatic hydrocarbons by microorganisms. I Enzymatic formation of catechol from benzene". Biochemistry. 7 (7): 2653–62. doi:10.1021/bi00847a031. PMID 4298226.
