Bernacchi is a surname, and may refer to:
- Antonio Maria Bernacchi, Italian castrato singer (1685-1756)
- Clelia Bernacchi , Italian actress and dubber (1910-2006)
- Louis Bernacchi, physicist, astronomer and explorer (1876-1942)
- Piero Bernacchi , Italian journalist and presenter
- Quirico Bernacchi, Italian cyclist (1914-2006)