Biss is a surname. Notable people with the surname include:
- Hubert E. J. Biss (1871-1909), British physician and medical writer
- Edwin Gerald Jones Biss (1876–1922), English motoring journalist and author
- Stan Biss (1892–1952), English greyhound trainer
- Józef Biss (1913-1977), Polish war criminal
- Harry Biss (1919-1997), American jazz pianist
- Earl Biss (1947-1998), Apsáalooke painter
- Daniel Biss (born 1977), American mathematician and politician
- Eula Biss (born 1977), American non-fiction writer
- Emma Biss (born 1990), Australian cricketer
- Tom Biss (born 1993), New Zealand footballer
- Benjamin Biss, musician