Bufalo can refer to:
- Búfalo, brand of hot sauce
- Bufalo pistol from Llama firearms
- Gaspare del Bufalo, a Roman Catholic priest and the founder of the Missionaries of the Precious Blood
- Bufalo Bill, an album of Italian singer-songwriter Francesco De Gregori.
- DN-V Bufalo, a Mexican self-propelled 75 mm howitzer
- Ciego de Avila (basketball), a Cuban basketball team nicknamed "Búfalos"