Wikipedia

CCDC186

CCDC186 is a protein that in humans is encoded by the CCDC186 gene[5] The CCDC186 gene is also known as the CTCL-tumor associated antigen with accession number NM_018017.[6]

CCDC186
Identifiers
AliasesCCDC186, C10orf118, coiled-coil domain containing 186, CCCP-1, golgin104, CCCP1
External IDsMGI: 2445022; HomoloGene: 9963; GeneCards: CCDC186; OMA:CCDC186 - orthologs
Orthologs
SpeciesHumanMouse
Entrez

55088

213993

Ensembl

ENSG00000165813

ENSMUSG00000035173

UniProt

Q7Z3E2

Q8C9S4

RefSeq (mRNA)

NM_018017
NM_001321829
NM_153249

NM_170757

RefSeq (protein)

NP_001308758
NP_060487
NP_694981

NP_739563

Location (UCSC)Chr 10: 114.12 – 114.17 MbChr 19: 56.78 – 56.81 Mb
PubMed search[3][4]
Wikidata
View/Edit HumanView/Edit Mouse

Gene

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Location

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CCDC186 has the chromosome location of 10q25.3 and is 53,750 bases in size oriented on the minus strand. PSORTII Protein k-NN Prediction indicated that C10orf118 is 65.2% of the time nuclear, 17.4% cytosolic, 8.7% mitochondrial, 4.3% vesicles of secretory system, and 4.3% endoplasmic reticulum.[7]

Expression

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Analysis of gene expression in humans and other species indicates C10orf118 is ubiquitously expressed in all tissue types at varying developmental stages. An EST profile from NCBI displayed the greatest expression in bone marrow, kidneys, and the prostate cell lines. Breakdown by health state indicates high expression of C10orf118 in bladder carcinoma and prostate cancer.[8]

Protein

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General Properties

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The protein of CCDC186 (NP_060487) is 898 amino acids in length. The predicted molecular weight is 103.7kdal and the isoelectric point is predicted to be 5.92.[9]

Composition

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A serine rich region is observed in amino acids 710-747. A compositional analysis revealed that C10orf118 is Proline (1.1%) poor and Glutamic acid (14.1%) and Lysine (12.0%) rich.[9]

Interactions

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CCDC186 protein was found to interact with proteins PLEKAH5, Ezra, GAMMAHV.ORF23, and SMAD3.[10][11]

Homology

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Orthologous sequences of CCDC186 were not found to be in bacteria, archea, protist, or plants. CCDC186 has no human paralogs. The date of divergence for the orthologous sequences highly correlates with the sequences similarity in that the percent identity decreases as you go back in time. Closely related orthologs include mammals and birds and moderately related orthologs include other vertebrates such as fish, reptiles, and amphibians. Distantly related orthologous sequences are primarily observed in invertebrates.[9][12]

Sequence Number Genus and species Common name Date of divergence (MYA) Accession number Sequence length Sequence identity Sequence similarity E-value
1 Homo sapiens C10orf118 0 NM_018017 898 aa 100% 100% 0%
2 Nomascus leucogenys Northern White-cheeked Gibbon 19.9 XP_003255542 898 aa 98% 98% 0%
3 Chinchilla lanigera long tailed chinchilla 90.9 XP_013371303 906 aa 90% 94% 0%
4 Mus musculus house mouse 90.9 NP_739563 917 aa 84% 91% 0%
5 Tursiops truncatus bottlense dolphin 97.5 XP_004318425 599 aa 60% 68% 0%
6 Loxodonta africana African Bush Elephant 105 XP_010596062 805 aa 90% 91% 0%
7 Egretta garzetta little egret 320.5 XP_009634185 914 aa 75% 83% 0%
9 Chelonia mydas geen sea turtle 320.5 XP_007056391 920 aa 76% 83% 0%
10 Xenopus tropicalis western clawed frog 355.7 XP_004919435 878 aa 71% 83% 0%
11 Astyanax mexicanus Mexican tetra 429.6 XP_007259323 987 aa 71% 82% 5.00E-09
12 Oryzias latipes Japanese rice fish 429.6 XP_011486278 991 aa 58% 71% 0%
13 Callorhinchus milii Australian ghostshark 482.9 XP_007903851 931 aa 65% 79% 0%
14 Strongylocentrotus purpuratus Sea Urchin 747.8 XP_011675415 884 aa 39% 58% 7.00E-84
15 Octopus bimaculoides California Two-spot Octopus 847 XP_014782587 1221 aa 42% 60% 3.00E-127
16 Aplysiomorpha sea hare 847 Not found in BLAST 1384 aa 42% 63% 3.00E-145
17 Orussus abietinus wasp 847 XP_012288999 1057 aa 36% 55% 5.00E-100
18 Atta cephalotes leafcutter ant 847 XP_012054311 1154 aa 35% 55% 9.00E-93
19 Helobdella robusta leech 847 ESO07814 997 aa 29% 47% 1.00E-50
20 Caenorhabditis elegans nematode 847 NP_871700 743 aa 26% 34% 1.00E-25
21 Trichoplax adhaerens NA 936 EDV25816 976 aa 26% 46% 2.00E-11

Motifs

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Motif Information[13] Position(s)
N-glycosylation site 33-36, 40-43, 109-112, 867-870
cAMP- and cGMP-dependent protein kinase phosphorylation site. 238-241, 583-586, 794-797
Casein kinase II phosphorylation site. 2-5, 10-13, 31-34, 36-39, 126-129, 130-133, 135-138, 139-142, 157-160, 228-231, 241-244, 256-259, 405-408, 440-443, 459-462, 490-493, 515-518, 611-614, 617-620, 625-628, 671-674, 690-693, 734-737, 869-872, 879-882
Leucine Zipper Pattern 588-609, 870-891
N-myristoylation site 32-37, 75-80, 86-91, 183-188, 721-726, 728-733, 745-750, 810-815, 828-833
Protein kinase C phosphorylation site 12-14, 35-37, 49-51, 146-148, 228-230, 243-245, 250-252, 278-280, 347-349, 371-373, 405-407, 417-419, 490-492, 500-502, 581-583, 592-594, 596-598, 682-684, 690-692, 825-827, 869-871, 895-897
Tyrosine kinase phosphorylation site 134-141, 514-522
Cullen Family Profile 443-552
K-Box Domain Profile 526-619
Nebulin Repeat Profile 521-531
Serine Rich Region 710-747
Rad50 zinc-hook domain profile 460-562
ATP synthase B/B' CF(0) 405-433
Alpha-2-macroglobulin RAP, C-terminal domain 524-705
Tropomyosin 334-577

Post Translational Modification

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CCDC186 is predicted to undergo multiple posttranslational modifications including predicted O-beta-GlcNAc attachment, phosphorylation, a nuclear export signal, glycation of lysines, GlcNAc O-glycosylation, N-glycosylation, and NetCorona sites.[14]

Clinical significance

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Prior research indicates that the open reading frame of C10orf118 is linked to cutaneous T-cell lymphoma by a tumor antigen L14-2.[15] The protein CCDC186 is also found at higher than normal levels in the breast cancer cell line BC 8701.[16]

References

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  1. ^ a b c GRCh38: Ensembl release 89: ENSG00000165813 – Ensembl, May 2017
  2. ^ a b c GRCm38: Ensembl release 89: ENSMUSG00000035173 – Ensembl, May 2017
  3. ^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  4. ^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  5. ^ Oduru S, Campbell JL, Karri S, Hendry WJ, Khan SA, Williams SC (June 2003). "Gene discovery in the hamster: a comparative genomics approach for gene annotation by sequencing of hamster testis cDNAs". BMC Genomics. 4 (1): 22. doi:10.1186/1471-2164-4-22. PMC 161800. PMID 12783626.
  6. ^ "Entrez Gene: C10orf118 chromosome 10 open reading frame 118".
  7. ^ "PSORT II Prediction". psort.hgc.jp. Retrieved 2016-04-24.
  8. ^ "Home - UniGene - NCBI". www.ncbi.nlm.nih.gov. Retrieved 2016-04-24.
  9. ^ a b c "SDSC Biology Workbench". seqtool.sdsc.edu. Archived from the original on 2003-08-11. Retrieved 2016-04-24.
  10. ^ "* in Literature citations". www.uniprot.org. Retrieved 2016-04-24.
  11. ^ "CCDC186 - Coiled-coil domain-containing protein 186 - Homo sapiens (Human) - CCDC186 gene & protein".
  12. ^ "BLAST: Basic Local Alignment Search Tool". blast.ncbi.nlm.nih.gov. Retrieved 2016-04-24.
  13. ^ "Motif Scan". myhits.isb-sib.ch. Retrieved 2016-05-08.
  14. ^ "Welcome to CBS". www.cbs.dtu.dk. Retrieved 2016-04-24.
  15. ^ de la Peña M, García-Robles I (September 2010). "Intronic hammerhead ribozymes are ultraconserved in the human genome". EMBO Reports. 11 (9): 711–6. doi:10.1038/embor.2010.100. PMC 2933863. PMID 20651741.
  16. ^ D’Angelo, M. L. (2013). "Identification of the "Uncharacterized Protein C10orf118" in Breast Cancer Cells and Its Role on the Hyaluronan Metabolism". Matrix Pathobiology, Signaling and Molecular Targets.
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Further reading

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